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10ug
雌激素受体α蛋白,ERRa Protein
Product Name:
human ESRRA-GST fusion protein
Catalog No:
Ag24631
Source:
E. coli-derived, PGEX-4T
Peptide Sequence:
MSSQVVGIEPLYIKAEPASPDSPKGSSETETEPPVALAPGPAPTRCLPGHKEEEDGEGAGPGEQG
(1-65 aa encoded by BC092470)
(1-65 aa encoded by BC092470)
Activity:
Not tested.
Endotoxin Level:
Please contact the lab for more information.
Purity:
95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
Formulation:
The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
Reconstitution and Storage
Reconstitution:
Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Shipping:
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).
Stability and Storage:
Store for up to 12 months at -20°C to -80°C as lyophilized powder.
Storage of Reconstituted Protein:
Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
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文献和实验相关实验
character. The N- and C- terminal and T-groups of the polypeptide can be ionized, contributing to the overall charge. The protein's net electric charge is the sum of the electric charges found on the surface of the molecule as a function of the environment
Protein Purification and Analysis
Levels of Structure in Protein Primary: A description of all covalent bonds. The sequence of AA residues Secondary: particularly stable arrangements of AA giving rise to recurring structural
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雌激素受体α蛋白,ERRa Protein
¥1500







