E-Cadherin(钙粘附蛋白E)

分子别名（Synonym）

Cadherin-1,CDH1,CD324,E-cadherin,Arc-1,ECAD,LCAM,UVO

表达区间及表达系统（Source）

Human E-Cadherin, Fc Tag (ECD-H5250) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Pro 621 (Accession # AAI41839).

Predicted N-terminus: Gln 23

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蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 92.4 kDa. The protein migrates as 100 kDa and 120 kDa Cadherin, a 48 kDa Propeptide under reducing (R) condition (SDS-PAGE) due to different glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human E-Cadherin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

文献引用（Citations）

• PAK2 links cell survival to mechanotransduction and metabolism

  Authors: Campbell HK, et al

  Journal: J Cell Biol 2019

  Application: Magnetic bead force assays & 3D atomic force microscopy

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• An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins.

  Authors: Eshghi A, et al.

  Journal: Cell Microbiol 2018

  Application: SPR

  Request for Full-text

• E-cadherin mediated lateral interactions between neighbor cells necessary for collective migration

  Authors: Kevin Suffoletto, et al.

  Journal: J Biomech 2018

前沿进展

• SARNP, a participant in mRNA splicing and export, negatively regulates E-cadherin expression via interaction with pinin
  Author : Kang Gyeoung Jin GJ, Park Mi Kyung MK, Byun Hyun Jung HJ, Kim Hyun Ji HJ, Kim Eun Ji EJ, Yu Lu L, Kim Boram B, Shim Jae Gal JG, Lee Ho H
  Journal : J. Cell. Physiol. 2019-07-17 DOI : 10.1002/smll.201802403
  Abstract : + Triple-negative breast cancer (TNBC) is associated with a high mortality rate, which is related to the insufficient number of appropriate biomarkers and targets.
• Galectin-1 induces metastasis and epithelial-mesenchymal transition (EMT) in human ovarian cancer cells via activation of the MAPK JNK/p38 signalling pathway
  Author : Zhu Jie J, Zheng Ya Y, Zhang Haiyan H, Liu Yanmei Y, Sun Hong H
  Journal : Am J Transl Res 2019-06-15 3862-3878 6 11 DOI : 10.1002/smll.201802403
  Abstract : + It has been reported that Galectin-1 (Gal-1) indicates bad prognosis of patients with ovarian cancer, and Gal-1 overexpression promotes metastasis of ovarian cancer cells.
• Anti-metastasis traditional Chinese medicine monomer screening system based on perinucleolar compartment analysis in hepatocellular carcinoma cells
  Author : Liu Feifei F, Lou Guohua G, Zhang Tianbao T, Chen Senzhong S, Xu Jia J, Xu Lichen L, Huang Chunhong C, Liu Yanning Y
  Journal : Am J Transl Res 2019-06-15 3555-3566 6 11 DOI : 10.1002/smll.201802403
  Abstract : + Hepatocellular Carcinoma (HCC) lacks effective anti-metastasis drugs.