调节T细胞磁珠分选,CD4 + CD25 + Regulatory T Cell Isolation Kit , human

调节T细胞磁珠分选,CD4 + CD25 + Regulat

ory T Cell Isolation Kit , human
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  • ¥11981
  • 美天妮
  • 130-091-301
  • USA
  • 2025年11月17日
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    • 询价记录
    • 技术资料
    • 英文名：

      CD4 + CD25 + Regulatory T Cell Isolation Kit , human

    • 保存条件：

      低温

    • 规格：

      for 1×10 9 total cells

    调节T细胞磁珠分选,CD4 + CD25 + Regulatory T Cell Isolation Kit , human

    CD4
    +
    CD25
    +
    Regulatory T Cell Isolation Kit
    , human
    Order no: 130-091-301

    CD4
    +
    CD25
    +
    Regulatory T Cell Isolation Kit
    , human
    For research use only
    Size: for 1×
    10
    9
    total cells
    The CD4
    +
    CD25
    +
    Regulatory T Cell Isolation Kit was developed for the isolation of CD4
    +
    CD25
    +
    cells from human PBMCs. The isolation is performed in a two-step procedure.

    Background information

    Regulatory CD4
    +
    CD25
    +
    T cells are suppressor cells that neutralize other immune cells by various mechanisms. Their characteristic marker is the transcription factor FoxP3. Regulatory CD4
    +
    CD25
    +
    T cells seem to suppress harmful immunological reactions to self or foreign antigens.

    Detailed separation procedure

    The isolation is performed in a two-step procedure. First, non-CD4
    +
    T cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies against CD8, CD14, CD15, CD16, CD19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A) and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted over a MACS® Column. In the second step, the flow-through fraction of pre-enriched CD4
    +
    T cells is labeled with CD25 MicroBeads for subsequent positive selection of CD4
    +
    CD25
    +
    regulatory T cells.

    Downstream applications

    The CD4
    +
    CD25
    +
    Regulatory T Cell Isolation Kit has been used to investigate the role of CD4
    +
    CD25
    +
    regulatory T cells in autoimmune diseases
    1,2
    , antitumor immunity
    3–6
    , allergy
    7
    , and in infectious diseases
    8
    . For further characterization, CD4
    +
    CD25
    +
    regulatory T cells were isolated from patients suffering from rheumatoid arthritis
    1
    or multiple sclerosis
    2
    , from acute T cell lymphoma patients
    4
    , from tumor biopsy specimens of non-Hodgkin lymphoma patients
    5
    , after dendritic cell vaccination
    9
    as well as from patients with atopic dermatitis
    7
    .

    Columns

    For the first magnetic separation (depletion): LD or autoMACS
    ®
    Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
    请选择指标和细胞
    产品编号 产品描述 规格 细胞上样量 分选方式
    人
    130-091-301 CD4+CD25+ Regulatory T Cell Isolation Kit, human hot! kit For 109 total cells 先去除分选，再阳性分选
    130-093-631 CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit, human kit For 2x109 total cells 先去除分选，再阳性分选
    130-094-775 CD4+CD25+CD127dim/- Regulatory T Cell Isolation Kit II, human hot! kit For 109 total cells 先去除分选，再阳性分选
    130-094-551 CD25+CD49d– Regulatory T Cell Isolation Kit, human kit For 109 total cells 先去除分选，再阳性分选
    MAGH104 MagCellect Human CD4+ CD25+ Regulatory T Cell Isolation Kit kit 1 x 109 total cells 先阴性分选，再阳性分选
    小鼠
    130-091-041 CD4+CD25+ Regulatory T Cell Isolation Kit, mouse hot! kit For 109 total cells 先去除分选，再阳性分选
    MAGM208 MagCellect Mouse CD4+ CD25+ Regulatory T Cell Isolation Kit kit 1 x 109 total cells 先阴性分选，再阳性分选
    非人灵长
    130-092-984 CD4+CD25+ Regulatory T Cell Isolation Kit, non-human primate kit For 109 total cells 先去除分选，再阳性分选

    分选器选择

    分选方式 产品名称 分选器产品编号 适配分选柱
    手动分选 MiniMACS Starting Kit 130-090-312 MS
    MidiMACS Starting Kit 130-042-301 LS/LD
    Mini&midiMACS Starting Kit 130-042-501 MS/LS/LD
    OctoMACS Starting Kit 130-042-108 MS
    QuadroMACS Starting Kit 130-091-051 LS/LD
    自动分选 Multi 24 MACS Starting Kit 130-098-637 AutoMACS Column
    autoMACS Starting Kit 130-092-545 AutoMACS Column

    分选柱选择

    分选方式 分选柱 分选柱产品编号 最大上样量 吸附目的细胞量
    手动分选 MS Columns 130-042-201 2x108 107
    LS Columns 130-042-401 2x109 108
    LD Columns（强阳性去除） 130-042-901 5x108 2x108
    Whool Blood Columns 130-093-545 Up to 15 mL whole blood
    自动分选 AutoMACS Column 130-021-101 4x109 2x108

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