CD4+CD25+Regulatory T Cell Iso

Polyclonal Expansion of Human CD4+CD25+ Regulatory T Cells

for the efficient in vitro expansion of human Treg cells and identified CD4+ CD25high CD45RA+ T cells as the ideal starting population for the generation of homogeneous and stable Treg cell products. Here, we provide detailed instructions for their identification

Regulatory T-Cells in Antitumor Therapy Isolation and Functional Testing of CD4+CD25+Regulatory T-Cells

for isolation of human as well as murine regulatory T-cells. A combination of positive and negative selection steps using immunomagnetic beads of different sizes yields preparations of functional, active CD4+ CD25+ regulatory T-cells with high purity

Human CD4+CD25highCD127low/neg Regulatory T Cells

CD4+CD25high CD127low/neg regulatory T cells (Tregs) play a critical role in the maintenance of peripheral tolerance and in controlling the development of autoimmune diseases. A combination of surface and intracellular markers, namely, CD25