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- BIO-RAD
- 3555797
- 2025年11月18日
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Pkg of 6, 225 ml bottles
Use Fraser 1/2 Broth (Demi-Fraser Broth) as a selective enrichment medium for the growth of Listeria in products intended for human and animal consumption. This broth can be used as a primary enrichment step for the identification and enumeration using RAPID'L.mono or RAPID'Listeria spp. Agar Plates.
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文献和实验in methanol at room temp. 3. Remove methanol and add 1x Blotting buffer until ready to use. G. Membrane transfer 1. Assemble "sandwich" for Bio-Rad's Transblot. 2. Prewet the sponges, filter papers (slightly bigger than gel) in 1x Blotting buffer
the bottle for contamination before you use it by gently swirling it and looking for cloudy material in the center. Always grow up a small amount of broth alone when growing cells overnight. A small amount of contamination is not always evident
, or BCA) (We use the Bradford assay from Bio-Rad.) Take x µl (= y µg protein) and mix with x µl of 2x sample buffer. Boil for 5 min. Cool at RT for 5 min. Flash spin to bring down condensation prior to loading gel.
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