NEK9丝氨酸/苏氨酸蛋白激酶NEK9抗体

NEK9丝氨酸/苏氨酸蛋白激酶NEK9抗体

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  • ¥1380 - 2200
  • LMAI Bio
  • LM-9317R
  • 进口/国产
  • 2025年11月26日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 (石蜡切片需做抗原修复)
  • Rabbit
  • Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
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    • 详细信息
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-9317R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      NEK9

    • 抗体名

      丝氨酸/苏氨酸蛋白激酶NEK9抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,

    • 免疫原

      KLH conjugated synthetic peptide derived from human NEK9:531-630/979

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      100ul  200ul

    NEK9丝氨酸/苏氨酸蛋白激酶NEK9抗体
    英文名称 NEK9
    中文名称 丝氨酸/苏氨酸蛋白激酶NEK9抗体
    别    名 Nek 8; NEK 9; Nek8; nek9; Nek-8; nek-9; NEK9_HUMAN; NERCC 1; Nercc 1 kinase; NERCC; NERCC1; Nercc1 kinase; Never in mitosis A related kinase 9; Never in mitosis A-related kinase 9; NIMA (never in mitosis gene a) related kinase 9; NIMA related kinase 8; NIMA related kinase 9; NIMA related kinase Nek 8; NIMA related kinase Nek 9; NIMA related kinase Nek8; NIMA related kinase Nek9; NimA related protein kinase 9; NIMA-related kinase 8; NimA-related protein kinase 9; Serine/threonine protein kinase Nek 9; Serine/threonine protein kinase Nek9; Serine/threonine-protein kinase Nek9.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 细胞生物  信号转导  细胞周期蛋白  激酶和磷酸酶  细胞分化  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 107kDa
    细胞定位 细胞核 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human NEK9:531-630/979 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.

    Function:
    Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively. 

    Subunit:
    Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP. Interacts with NEK6, NEK7 and BICD2. Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex.

    Subcellular Location:
    Cytoplasm. Nucleus.

    Tissue Specificity:
    Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.

    Post-translational modifications:
    Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro. [SIMILARITY] Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. [SIMILARITY] Contains 1 protein kinase domain. 

    Similarity:
    Contains 6 RCC1 repeats.

    SWISS:
    Q8TD19

    Gene ID:
    91754

    Database links:

    Entrez Gene: 91754 Human

    Entrez Gene: 217718 Mouse

    Entrez Gene: 299204 Rat

    Omim: 609798 Human

    SwissProt: Q8TD19 Human

    SwissProt: Q8K1R7 Mouse

    Unigene: 7200 Human

    Unigene: 29071 Mouse

    Unigene: 102369 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 NEK9丝氨酸/苏氨酸蛋白激酶NEK9抗体
    Tissue/cell: Mouse testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-NEK9 Polyclonal Antibody, Unconjugated(bs-9317R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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