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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-9135R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
ASB17
- 抗体名:
含锚蛋白重复序列-细胞因子信号抑制物盒蛋白家族17抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human ASB17:81-180/295
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | ASB17 |
| 中文名称 | 含锚蛋白重复序列-细胞因子信号抑制物盒蛋白家族17抗体 |
| 别 名 | Ankyrin repeat and SOCS box containing 17; Ankyrin repeat and SOCS box protein 17; ASB-17; ASB17; ASB17_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 34kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ASB17:81-180/295 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Members of the suppressor of cytokine signaling (SOCS) family of proteins contain C-terminal regions of homology called the SOCS box, which serves to couple SOCS proteins and their binding partners with the Elongin B and C complex. Several other families of proteins also contain SOCS boxes, but differ from the SOCS proteins in the type of domain they contain upstream of the SOCS box. The largest family of SOCS box-containing proteins is the ankyrin repeat and SOCS box-containing (ASB) protein family. Members of the ASB family include ASB-1 through ASB-18 and are involved in a variety of biological processes. ASB-17 is a 295 amino acid member of this family. ASB-17 contains one ankyrin repeat and one SOCS box domain. ASB-17 is thought to be a substrate-recognition subunit of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin protein ligase complex. This complex mediates the ubiquitination of target proteins and their subsequent proteasomal degradation. Function: May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Similarity: Contains 1 ANK repeat. Contains 1 SOCS box domain. SWISS: Q8WXJ9 Gene ID: 127247 Database links: Entrez Gene: 127247 Human Entrez Gene: 66772 Mouse SwissProt: Q8WXJ9 Human SwissProt: Q8VHP9 Mouse Unigene: 125423 Human Unigene: 116960 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验淋巴母细胞可分泌高水平TNF-β。 2.TNF的分子结构和基因 (1)人的TNF-α基因长约2.76kb,小鼠为2.78kb,结构非常相似, 均由4个外显子和3个内含子组成,与MHC基因群密切连锁,分别定位于第6对和第17对染色体上。1984年从HL—60、U937等细胞中克隆成功rHuTNF-α cDNA,并在大肠杆菌中获得高表达。 人TNF-α前体由233个氨基酸残基组成,含76个氨基酸残基的信号肽,切除信号肽后成熟型TNF-α为157氨基酸残基,非糖基化,第69位和10l位两个半胱
,并在大肠杆菌中获得高表达。 人TNF-α前体由233个氨基酸残基组成,含76个氨基酸残基的信号肽,切除信号肽后成熟型TNF-α为157氨基酸残基,非糖基化,第69位和101位两个半胱氨酸形成分子内二硫键。rHu TNF-α分子量为17kDa。小鼠TNF-α前体为235氨基酸残基,信号肽79氨基酸残基,成熟的小鼠TNF-α(rMuTNF-α)分子量为17kDa,由156个氨基酸残基组成,第69位和100位两个半胱氨酸形成分子内二硫键,有一个糖基化点,但糖基化不影响其生物学功能。rHu TNF-α与rMu
。该方法可用于含重复序列及较长序列的DNA序列测定及不同基因组同源区域的序列比较。利用基因芯片测序的准确率达99%以上。 正如NIH首脑Harold Varmus在美国细胞生物学1998年年会上所说:“在基因芯片的帮助下,我们将能够监测一个细胞乃至整个组织中所有基因的行为。”(二)基因型、基因突变和多态性分析 在同一物种不同种群和个体之间,有着多种不同的基因型,而这种不同,往往与个体的不同性状和多种遗传性疾病有着密切的关系。通过对大量具有不同性状的个体的基因型进行比较,就可以得出基因与性状的关系
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