Mouse IgG2a Isotype Control SA

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

Immunofluorescent Staining of Mouse and Rat Leukocytes

. Examples of negative controls are unstained cells, cells exposed to the 2nd step reagent alone, or cells exposed to isotype controls which are the same isotype and format (e.g.,purified, biotin or fluorochrome) as the primary antibody and titrated

BAC Transgenes, DNA Purification, and Transgenic Mouse Production

by the microinjection of purified DNA into the pronuclei of fertilized mouse eggs in both cases. Despite this similarity, the application of small DNA injection methods to large DNA transgenes leads to the integration of DNA fragments instead of intact BAC transgenes