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文献和实验Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
) (4 ). Phage Ab repertoires can be subjected to multiple rounds of panning on individual immobilized antigens (Ags) in order to isolate individual Ag-binding clones. This approach has been successfully applied to numerous purified soluble molecules, yielding
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
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