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- 详细信息
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- 文献和实验
- 技术资料
- 抗体英文名:
JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody
- 靶点:
JNK1/JNK2/JNK3
- 浓度:
1.0mg/ml
- 应用范围:
WB IF
- 宿主:
Rabbit
- 适应物种:
Hu Ms Rt
- 克隆性:
多克隆
- 保存条件:
Store at -20℃
- 形态:
liquid
- 免疫原:
Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V ) derived from Human JNK1/JNK2/JNK3.
- 规格:
100ul
Purification:
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Specificity:
The antibody detects endogenous level of JNK1/JNK2/JNK3 only when phosphorylated at Thr183/Tyr185.
Immunogen Description:
Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V ) derived from Human JNK1/JNK2/JNK3.
Target Name:JNK1/JNK2/JNK3
Modification:Phospho
Alternative Names:
Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; JNK-46
Accession No.:
Swiss-Prot: P45983 P45984 P53779
NCBI Protein: NP_002741.1 NP_001128516.1 NP_002744.1
Concentration: 1.0mg/ml
Formulation :
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Images

Western blot analysis of extracts from C6 cells untreated or treated with anisomycin using JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody #11504.

Immunofluorescence staining of methanol-fixed Hela cells using JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody #11504.

Western blot analysis of extracts from 293 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using JNK1/JNK2/JNK3 (phospho-Thr183/Tyr185) Antibody #11504.
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运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
results of phosphorylated proteins磷酸化的试剂盒中的抗体是经过大量筛选,选取了高特异性和低背景的抗体。p-P38MAPK抗体是基于P38的磷酸化Thr180到Tyr182这个位点多肽片段所制备。P-JNK抗体是基于人的Thr183和Tyr185.这个抗体可以同时识别P-JNK1和P-JNK2.我们在运用Cell Based Elisa P38和JNK两个试剂盒做检测的同时,又用传统的WB方式来验证这个结果。经过反复验证,都得出了同样的结果, Cell Based
) is accompanied with or inactivates the GSK activity. However, it is still not conclusive what the role of Tyr phosphorylation (Y216 fro beta, Y279 for alpha) on GSK activity is. One reference is a review paper published in Cell in 2001, you may take a quick
secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total













