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PP2A alpha + beta蛋白质磷酸酶2A (alp

ha + beta)抗体
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  • ¥1380 - 2200
  • LMAI Bio
  • LM-6706R
  • 进口/国产
  • 2025年12月30日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
  • Rabbit
  • Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit,
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-6706R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      PP2A alpha + beta

    • 抗体名

      蛋白质磷酸酶2A (alpha + beta)抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit,

    • 免疫原

      KLH conjugated synthetic peptide derived from human PP2A beta/PPP2CB:21-120/309

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      100ul  200ul

    PP2A alpha + beta蛋白质磷酸酶2A (alpha + beta)抗体
    英文名称 PP2A alpha + beta
    中文名称 蛋白质磷酸酶2A (alpha + beta)抗体
    别    名 Medium tumour antigen associated 61kDa protein; MGC786; PP2A beta; PP2A PR65 alpha isoform; PP2A-beta; PP2AB_HUMAN; PP2Abeta; PP2CB; Ppp2cb; Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; Protein phosphatase 2 catalytic subunit beta isozyme; Protein phosphatase 2 regulatory subunit A alpha isoform; Protein phosphatase 2, catalytic subunit, beta isoform; Protein phosphatase 2A catalytic subunit beta isoform; Protein phosphatase type 2A catalytic subunit; Serine threonine protein phosphatase 65 kDa; Serine/threonine protein phosphatase 2A catalytic subunit beta; Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform.  PP2A β; PP2A-β; PP2Aβ; PP2Aα; PP2A-Cα/β; PP2A-C α/β; PP2A-C α + β; PP2A-C α+β.
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 细胞生物  信号转导  细胞周期蛋白  激酶和磷酸酶  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 36kDa
    细胞定位 细胞核 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human PP2A beta/PPP2CB:21-120/309 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]. 

    Function:
    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.

    Subunit:
    Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with TBCD (By similarity). PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1. May indirectly interact with SGOL1, most probably through regulatory B56 subunits. 

    Subcellular Location:
    Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.

    Post-translational modifications:
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro). 
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. 

    Similarity:
    Belongs to the PPP phosphatase family. PP-1 subfamily. 

    SWISS:
    P62714

    Gene ID:
    5515

    Database links:

    Entrez Gene: 5515 Human

    Entrez Gene: 5516 Human

    Entrez Gene: 19052 Mouse

    Entrez Gene: 19053 Mouse

    Entrez Gene: 24672 Rat

    Entrez Gene: 24673 Rat

    Omim: 176915 Human

    Omim: 176916 Human

    SwissProt: P62714 Human

    SwissProt: P67775 Human

    SwissProt: P62715 Mouse

    SwissProt: P63330 Mouse

    SwissProt: P62716 Rat

    SwissProt: P63331 Rat

    Unigene: 105818 Human

    Unigene: 491440 Human

    Unigene: 260288 Mouse

    Unigene: 288765 Mouse

    Unigene: 1271 Rat

    Unigene: 977 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 产品细节图片1
    Sample: Testis (Mouse) Lysate at 40 ug
    Primary: Anti-PP2A beta (bs-6706R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 36 kD
    Observed band size: 40 kD

    产品细节图片2
    Sample: Brain (Mouse) Lysate at 40 ug
    Primary: Anti-PP2A beta (bs-6706R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 36 kD
    Observed band size: 40 kD

    产品细节图片3
    Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PP2A beta) Polyclonal Antibody, Unconjugated (bs-6706R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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