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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-6133R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
WNT2
- 抗体名:
信号通路Wnt2抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Chicken, Cow, Rabbit,
- 免疫原:
KLH conjugated synthetic peptide derived from human WNT2:221-320/360
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
50ul 100ul 200ul
| 英文名称 | WNT2 |
| 中文名称 | 信号通路Wnt2抗体 |
| 别 名 | Int 1 related protein; IRP protein; WNT 2; INT1L 1; INT1L1; ONCOGENE INT1 LIKE 1; Wingless type MMTV integration site family member 2; WNT2_HUMAN. |
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Specific References (1) | bs-6133R has been referenced in 1 publications.
[IF=3.71] Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995 WB ; Dog.
PubMed:24404146
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| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 染色质和核信号 信号转导 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Cow, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNT2:221-320/360 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Subcellular Location: Secreted. Tissue Specificity: Expressed in brain in the thalamus, in fetal and adult lung and in placenta. Similarity: Belongs to the Wnt family. SWISS: P09544 Gene ID: 7472 Database links: Entrez Gene: 7472 Human Entrez Gene: 22413 Mouse Entrez Gene: 114487 Rat Omim: 147870 Human SwissProt: P09544 Human SwissProt: P21552 Mouse Unigene: 567356 Human Unigene: 33653 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti- WNT2 (bs-6133R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 37 kD Observed band size: 38 kD
Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.
Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.
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文献和实验。HSP以直接或间接的方式参与了细胞凋亡的调节,其作用靶点涉及不同凋亡信号通路的各主要激酶或关键性调控蛋白。除对凋亡 相关信号转导通路的蛋白质直接调节作用之外,还涉及了一些细胞膜生长因子受体,如EGFR、ErbB2、FLT3及细胞内雌、雄激素受体等蛋白质稳定性和 活性的调节,这种调节作用具有双向性。即既有抗凋亡的作用,也有促凋亡的作用。细胞凋亡分为线粒体通路介导的凋亡及死亡受体通路介导的凋亡,而HSP不同 程度地对这两条通路的活化具有调节作用。Hsp90能过阴断Apaf-1寡聚化,最终阴断凋亡体的形成
免疫研究新方法:IGF信号通路抗体芯片筛选出肝癌靶生物标志物
单一片面的,之前因为缺少检测工具,无法对 IGF 信号通路中的蛋白表达进行全面的检测,本文第一次采用了RayBiotech(QAH-IGF-1)抗体芯片对 25 对肝癌与正常组织进行高通量检测对比。 IGF-2R 和 IGFBP-2 在肝癌组织中提高。其他 8 个没有显著变化。这为肝癌药物研究和开发提供新的靶标。
受体阳性的乳腺癌细胞有较小程度的细胞毒性,这种情况与在异种移植小鼠模型中的表现一致。体外实验中,在 TNBC 细胞的诱导下,初始 EGFR CAR-T 迅速扩增,同时还会激活 interferomγ, granzyme-perforin-PARP 和 FaS-FADD-caspase 等相关信号通路。 实验团队首先通过免疫印迹和流式细胞术的方法验证 EGFR 高表达于三阴乳腺癌。其中免疫印迹(immunoblotting)又称蛋白质印迹(Western blotting),是根据抗原抗体的特异性结合检测
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