- ¥780 - 2200
- LMAI Bio
- LM-0173R
- 进口/国产
- 2025年07月15日
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-0173R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
IRS-2
- 抗体名：
胰岛素受体底物-2抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat,
- 免疫原：
KLH conjugated synthetic peptide derived from human IRS-2:501-600/1324
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
| 英文名称 | IRS-2 |
| 中文名称 | 胰岛素受体底物-2抗体 |
| 别 名 | Insulin Receptor Substrate 2; IRS 2; IRS-2; IRS2; IRS2_HUMAN. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞凋亡 细胞膜受体 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 146kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IRS-2:501-600/1324 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The family of insulin receptor substrates (IRSs) has been reported to play important roles for signal transduction of various hormones. Four members of the IRS family have been described. Each IRS is believed to have different functions; however, the distinct physiological roles of each IRS are unclear. Summary: This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. Function: May mediate the control of various cellular processes by insulin. Subunit: Interacts with PHIP (By similarity). Subcellular Location: Cytoplasm, cytosol. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 IRS-type PTB domain. Contains 1 PH domain. SWISS: Q9Y4H2 Gene ID: 8660 Database links: Entrez Gene: 8660 Human Entrez Gene: 384783 Mouse Entrez Gene: 29376 Rat Omim: 600797 Human SwissProt: Q9Y4H2 Human SwissProt: P81122 Mouse Unigene: 442344 Human Unigene: 407207 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. IRS-2是肝胰岛素信号转导核心介子，IRS-2基因的缺失或IRS-2信号网络上一些关键信号分子的异常改变，都将导致肝胰岛素信号转导能力减弱，出现肝胰岛素抵抗. |
| 产品图片 |  Tissue/cell: rat skeletal muscle; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IRS2 Polyclonal Antibody, Unconjugated(bs-0173R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control (black line): HepG2(black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary Antibody (Red line): Rabbit Anti-IRS-2 antibody (bs-0173R) ; Dilution: 1μg /10^6 cells; Isotype Control Antibody (green line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test. |
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文献和实验胰岛素受体底物(insulin receptor substrate，IRSs)
能够被激活的胰岛素受体酪氨酸激酶作用的底物, 其上具有十几个酪氨酸残基可被磷酸化，磷酸化的IRSs能够结合并激活下游效应物。 IRSs在被胰岛素受体磷酸化以后，如同一块“磁铁”与那些具有SH2结构域的蛋白结合，根据所结合蛋白的具体结构产生不同的效应，如激活SH2蛋白的酶活性、改变蛋白质构型并同另外的蛋白结合或者引起蛋白质从细胞的一个部位转移到另一个部位。 已知有三种胰岛素受体酪氨酸激酶作用的底物(IRSs)。第一种是胰岛素受体底物1(IRS1),是一种
尿病组胰岛素信号通路IRS-1位点是上调，而出现的p-akt 丝氨酸位点是下调表达，怎么解释这个现象呢？迷糊 米宝 Akt 和p-akt 全部做完了 哈哈 yangfeiya122 米宝 wrote: Akt 和p-akt 全部做完了 哈哈 您好！两者是什么趋势呢，是总的差不多，磷酸化的降低吗？谢谢！ 我最近也在做着两个，真的好难做啊！
证据显示这可能是继发于高胰岛素血症的结果，绝大多数胰岛素抵抗是胰岛素和胰岛素受体结合后信号传导过程发生障碍的结果，主要缺陷包括胰岛素受体的酪氨酸激酶活性下降、胰岛素信号传导异常、葡萄糖转运减少、葡萄糖磷酸化和糖原合成酶活性减弱等。其中糖原合成酶活性减弱是导致胰岛素抵抗的早期因素，可以在正常血糖或葡萄糖耐量受损的胰岛素抵抗患者的骨骼肌中发现。新近的研究表明，糖原合成酶活性降低是胰岛素受体磷酸化胰岛素受体底物-1（IRS-1）的能力下降，减弱了IRS1和PI3-K亚单位p85的结合，最终降低PI3K活性
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