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- 2025年12月05日
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1 mL/5 mL/30 mL/120 mL
| 规格： | 1 mL | 产品价格： | ￥210.0 |
|---|---|---|---|
| 规格： | 5 mL | 产品价格： | ￥680.0 |
| 规格： | 30 mL | 产品价格： | ￥2400.0 |
| 规格： | 120 mL | 产品价格： | ￥7680.0 |
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Cell Counting Kit-8
MCE 国际站：Cell Counting Kit-8
Cell Counting Kit-8，简称CCK-8试剂盒或CCK8试剂盒，是一种基于WST-8而广泛应用于细胞活性和细胞毒性检测的快速、高灵敏度试剂盒。
Description & Advantages:
Cell Counting Kit-8 (CCK-8) provides a tool for studying induction and inhibition of cell proliferation in any in vitro model. Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4- disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator.
CCK-8 is a one-bottle solution, ready to use. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays.
1. More Sensitive than MTT, MTS, or WST-1
2. No Toxicity to Cell
3. Simple Steps (No Thawing Necessary)
4. Stable One Bottle Solution : 1 year at 4°C
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文献和实验Overview Count the number of viable cells in a culture via dilution plating. The following assumes E. coli cells but it should apply to any type of cells. Materials LB agar plate LB media for dilution
Cell counting with an hemacytometer.
Inc. Garden grove CA 92841 Hemacytometer Fisher scientific No 02671 5 Dilute your cell sample in Trypan Blue dye exclusion medium. Dead cells will be blue, live cells will be birefringent. Suggested dilution 1:20 (5µl cell suspension in 95 µl Trypan Blue
Using a Counting Chamber For microbiology, cell culture, and many applications that require use of suspensions of cells it is necessary to determine cell concentration. One can often determine cell density of a suspension
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