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- 详细信息
- 技术资料
- 英文名:
Phusion High-Fidelity DNA Polymerase (2 U/µL)(pfu酶替代)
- 保存条件:
高保真 DNA 聚合酶 (2 U/μL)
- 供应商:
上海研卉生物科技有限公司
- 库存:
高保真 DNA 聚合酶 (2 U/μL)
- 规格:
500T
Phusion High-Fidelity DNA Polymerase (2 U/µL)(pfu酶替代)
Phusion™ 高保真 DNA 聚合酶 (2 U/μL)
• Phusion DNA 聚合酶 (2 U/μL)
• 5X Phusion HF 缓冲液
• 5X Phusion GC 缓冲液
• DMSO
• 50 mM MgCl2 溶液
Phusion HF 缓冲液和 Phusion GC 缓冲液都提供最终 1X 浓度的 1.5 mM MgCl2。
在-20°C 条件下储存。
描述
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.
Highlights
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)
Applications
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray
Thermo Scientific Phusion 高保真 DNA 聚合酶为高效 PCR 设立了“金标准”。Phusion 高保真 DNA 聚合酶的错误率是 Taq 的 1/50,是 Pfu 的 1/6,因此是克隆和其他需要高保真度的应用的极,佳之选。与其他 DNA 聚合酶相比,Phusion DNA 聚合酶性能稳健,实验方案耗时短(即使在含有 PCR 抑制剂的情况下也是如此),且能够以较少的酶量获得较高得率。
产品优势
•高保真度(52X Taq)
•延伸时间短,可实现快速 PCR (15-30 s/kb)
•性能稳健,仅需较少优化
•以较少的酶量获得高得率的 PCR 产物
•有 Green 缓冲液规格可供选择,用于将 PCR 产物直接上样至凝胶(F-534S 或 F-534L)
应用
•高保真 PCR
• 克隆
•生成测序模板
•扩增复杂(高 GC 含量)模板
•长片段 PCR(达 20 kb)
•诱变
•高通量 PCR
• 微阵列
规格
| Polymerase: | Phusion High-Fidelity DNA Polymerase |
|---|---|
| Hot Start: | No |
| Fidelity (vs. Taq): | 52 X |
| GC-Rich PCR Performance: | High |
| Reaction Format: | Standalone |
| Product Overhang: | Blunt |
| Reaction Speed: | Fast |
| Product Line: | Phusion |
| Product Size: | 100 units |
内容及储存
• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
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