Sigma货号E0412-1VL人羧基酯酶2(别名:人羧基酯

Sigma货号E0412-1VL人羧基酯酶2(别名:人羧基酯酶2，CES2，CES2A1)现货19121878899上海睿安生物科技有限公司Merck-Roche-Millipore-Sigma官方授权代理商

羧基酯酶2人

recombinant,expressed in baculovirus infected BTI insect cells

别名:羧基酯酶2人,CES2,CES2A1

属性

recombinant:expressed in baculovirus infected BTI insect cells

form:liquid

specific activity:≥500 units/mg protein

mol wt:60 kDa

weight:(0.5 ml)

shipped in:dry ice

storage temp:−70°C

Quality Level:200

说明

General description

羧酸酯酶2人类基因定位于人类染色体16q22.1。

羧酸酯酶属于α/β水解酶折叠家族，参与药物代谢和活化。存在于结肠、肝脏、小肠、心脏、脑和睾丸中。小肠的水解酶活性归因于人的羧酸酯酶2。它在103和267个残基处有两个糖基化位点，分子量为60kDa。

Application

人的羧酸酯酶2已用于:

• 在体外基于酶的抑制剂筛选测定
• 在以发射比率双光子探针(SE1)的底物选择性测定
• 在各种基于4-硝基(jī)苯基和1-萘基的底物的酶测定中作为标准品

具有高催化活性，非常适合用于强大的高通量筛选分析，包括药物间相互作用研究以及用于评估前药和非CYP消除途径的药代动力学研究。

羧酸酯酶2可能在多种含酯药物和前体药物的生物转化中起关键作用。

Biochem/physiol Actions

人的羧酸酯酶2确定了含有大醇和小酰基的位点。由于在活性位点存在构象干扰，它显示出较少的酯交换活性。

可水解酯和酰胺键的丝氨酸酯酶家族成员。羧基酯酶2是一种内质网结合的水解酶，在异源生物解毒和活化含酯治疗剂中起关键作用。羧基酯酶2也可参与海(luò)因和可(kǎ)因等药物的解毒过程。该酶被认为在脂质代谢中起作用。

Other Notes:一个单位将在37℃、pH 7.4下每分钟从4-硝基(běn)乙(yǐ)酸酯生产1纳摩尔的4-硝(jī)(běn)酚。

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