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- 11388 25 MG
- 美国
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 保质期：
见产品说明书
- 保存条件：
见产品说明书
- CAS号：
见产品说明书
- 英文名：
APROTININ, ü6,000 KALLIKREIN
- 规格：
25 MG
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文献和实验/L EDTA, 2 mmol/L EGTA, 10 mmol/L sodium orthovanadate, 10 µg/L leupeptin, and 10 µg/L aprotinin). The homogenate was centrifuged at 30 000g for 30 minutes at 4°C. The pellet was resuspended in buffer B (buffer A with 1% NP-40), stirred for 2 hours
μg/ml pepstain A, 50 μg/ml leupeptin and 0.2% aprotinin] and allowed to swell on ice for 30 min.3. Cells were then frozen in a dry ice bath and thawed on ice.4. Cells were spun at 10 000 rpm for 10 min and the supernatant was frozen at -80
Isolation of cell nuclei for the application in the cell-free system
: resuspend in NSB at 1E+07 nuclei/ml and distribute the suspension in 5 µl aliqots (50 000 nuclei). Store nuclei at -70°C in aliquots until required (up to several weeks). ---------------------------------------------------------
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