逆转录酶SuperScript II，1万单位 Therm

货号： 18064014

 

规格

Optimal Reaction Temperature:	42° C
Final Product Size(s):	12.3 kb or less
Reverse Transcriptase:	SuperScript® II
Ribonuclease H Activity:	Reduced
Form:	Frozen
Format:	Tube(s)
Template:	ssDNA, ssRNA, RNA-DNA Hybrid
Sensitivity:	Medium
Product Size:	10,000 units
Concentration:	200 U⁄µl
Enzyme Function:	RNA dependent DNA Polymerase
Shipping Condition:	Wet Ice
Number of Reactions:	50 Reactions

 

内容及储存

SuperScript® II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl₂], and a vial of 100 mM DTT.

Store at -20°C. Guaranteed stable for 6 months when properly stored.

 

描述

SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics.

• Yield – Reduced RNase H activity results in greater first-strand cDNA yields
• Size of cDNA – Generation of RT-PCR products up to 12 kb
• Thermostability – Full activity at 42°C
• Flexibility – Uses either total or poly(A)⁺ RNA

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)_12-18 as template•primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl₂, 1 mM DTT, 0.5 mM [³H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)_12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.