SuperScript® III Reverse Trans

 

规格

Optimal Reaction Temperature:	50° C
Final Product Size(s):	12.3 kb or less
Reverse Transcriptase:	SuperScript® III
Ribonuclease H Activity:	Reduced
Form:	Frozen
Format:	Tube(s)
Quantity:	10,000 units
Template:	ssRNA
Sensitivity:	Medium
Product Size:	10,000 units
Concentration:	200 U⁄µl
Enzyme Function:	RNA dependent DNA Polymerase
Shipping Condition:	Dry Ice
Number of Reactions:	50 Reactions

 

内容及储存

SuperScript® III Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl₂], and a vial of 100 mM DTT.

Store at -20°C. Guaranteed stable for 6 months when properly stored.

 

描述

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR ofa specific gene or generating cDNA from total or poly (A)⁺ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.

• Thermostability – Half life of 220 minutes at 50°C
• Yield – Reduced RNase H activity for more full-length cDNA
• Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
• Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3´ and 5´ RACE

Source: Purified from E. coli expressing the pol gene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.
Unit Definition: One unit of SuperScript® III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)_12-18 as a template•primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl₂, 1 mM DTT, 0.5 mM [³H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)_12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Invitrogen™SuperScript™ III 逆转录酶18080044，产品简介:
产品品牌：Invitrogen
产品名称：Invitrogen™SuperScript™ III 逆转录酶
英文名称：Invitrogen™SuperScript™IIIReverseTranscriptase
产品货号：18080044
产品规格：10,000 units
Invitrogen™SuperScript™ III 逆转录酶18080044，产品说明：
Invitrogen SuperScript III 逆转录酶是经过基因改造的 MMLV 逆转录酶 (RT)，其产生方式是通过引入几种突变来降低 RNase H 活性、增加半衰期和改进热稳定性。相较于野生型 MMLV 和 MMLV RNase H- 酶，SuperScript III RT 具有更高的 cDNA 产量，改进的 cDNA 长度，更高的高GC含量 靶标 RNA 效率和整体更好的性能。
SuperScript RT 是高度值得信赖和广泛使用的 RT，迄今为止已有超过 50,000 次引用、评论和出版物。
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