XP beads 磁珠 A63880/A63881/A63882 Beckman 贝克曼 背景资料:The Agencourt AMPure XP system is a highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, Agencourt AMPure XP can be easily used in manual and automated 96- or 384-well formats.。 产品描述:Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NXP and FXP for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods.The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system. Application: PCR purification Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking 产品特点: 1.High recovery of amplicons, greater than 100 bp ; 2.Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants ; 3.Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days ; 4.Efficient recovery of double stranded and single stranded DNA templates ; 5.Consistent recovery throughout the kit’s 12 month shelf life ; 6.Faster manual and automated processing as compared to traditional post-PCR cleanup methods。 产品特性: • 200bp以上的核酸回收率高达90%以上,核酸长度最小要求100bp • 有效去除dNTP、引物、引物二聚体、盐离子和其它杂质 • 纯化后的产物在4℃温度下保持7天不被降解 • 双链和单链DNA都能得到纯化 • 长达18个月有效期 • 手工操作和自动化工作站操作皆可 操作过程: 1. 加入AMPure XP 磁珠试剂 2. 磁珠与核酸结合 3. 结合有核酸的磁珠与杂质分离 4. 乙醇洗涤 5. 加入洗脱液 6. 转移洗脱产物 应用领域: 该试剂盒应用于: PCR体系纯化、酶切和连接反应体系纯化
产物适合于下游应用:PCR、Genotyping(SNP detection)、sequencing(Sanger and Next Generation Sequencing)、Cloning、Primer Walking