Klenow大片段库存

特别声明：本产品及我公司所售其他产品均为科研类试剂产品，严禁用于药物、医疗及其他非科研用途。

北京百奥莱博专业生产供应Klenow大片段库存，我公司销*(代"售")全品类的分子生物学试剂，价格优惠，欢迎广大科研工作者垂询订购。

名称：Klenow大片段库存
编号：JN0070
规格：150U×5
品牌：百奥莱博
产地：北京
  本酶是大肠杆菌DNA聚合酶I经基因工程改造的产物。由于基因重组，其不含有DNA聚合酶I的5´→3´外切核酸酶活性，只具有5´→3´的DNA聚合酶活性和3´→5´的外切核酸酶活性。其中3´→5´的外切核酸酶的活性较弱，不适用于3´突出端的切平。本品浓度为5U/ul。

产品用途：
1、双脱氧法DNA序列测定。 (Sanger法)
2、突出端的补平。（参见实验方案）
3、使用随机引物进行DNA标记。

活性定义：
在37℃条件下，30分钟内能使10 nmol的dNTPs渗入酸不溶性沉淀物所需要的酶量定义为1个活性单位。

质量保证：经多次柱纯化，SDS-PAGE胶检测仅可见清晰单一的目的条带；PCR方法检测无大肠杆菌DNA残留，无核酸内、外切酶污染。

热失活：75℃，20分钟。

关于Klenow大片段库存的更详细说明，请致电我公司咨询，我们将以最饱满的热情为您提供解答，此外，我公司专业供应下列产品：

·重组人PGK1(磷酸甘油酸激酶1)
编号：JN1254
英文名称：Recombinant Human Phosphoglycerate kinase 1
规格：10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成，目的基因编码的Ser2-Ile417在C端含有His标签。

PGK1质量控制：＞95%(还原性SDS-PAGE)

PGK1制剂：液体

PGK1保存：收到货后请置于-20℃，可保存6个月，避免反复冻融。

关于PGK1：
PGK1 is called phosphoglycerate kinase that involved in a critical energy-producing process known as glycolysis. Phosphoglycerate kinase helps carry out a chemical reaction that converts a molecule called 1,3-diphosphoglycerate, which is produced during the breakdown of glucose, to another molecule called 3-phosphoglycerate during glycolysis. PGK1 The encoded protein may also act as a cofactor for polymerase alpha.. The protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions.

·重组人PD-L1(B7-H1)
编号：JN1815
英文名称：Recombinant Human Programmed Cell Death 1 Ligand 1
规格：10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成，目的基因编码的Phe19-Thr239在C端含有His标签。

PD-L1/B7-H1质量控制：＞95%(还原性SDS-PAGE)

PD-L1/B7-H1制剂：冻干品

PD-L1/B7-H1保存：
冻干蛋白置于-20℃以下可长期保存，室温条件下可稳定保存3周。
复溶蛋白溶液可在4～7℃保存2～7天，可分装后置于-20℃保存三个月。

PD-L1/B7-H1复溶：
打开试剂管前请先离心。
复溶浓度推荐大于100μg/ml。
冻干蛋白请溶于ddH2O。
复溶后，请根据用量分装冻存，避免反复冻融。

关于PD-L1/B7-H1：
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.


Klenow大片段库存关键词：Klenow大片段,百奥莱博,JN0070

BTN60805 中草药DNA提取试剂盒 Chinese herbal medicine DNA extraction kit
SY0225 RORα2-GFP报告基因质粒 RORα2 GFP Reporter Plasmid
RFT155 PAGE预制胶(8-16%)
KFS199 Caspase 3荧光法检测试剂盒(AFC) Caspase-3 Fluorescence Metric Assay
ALH187 2×Real-Time PCR预混液 2×Real Time PCR Mix
YT572 乙酸激酶 Acetate Kinase (Crude Enzyme)
BTN130898 MAL指示剂培养基 MAL Indicator Medium
HR0336 BSA标准 BSA standard
SV1320 microRNA Marker
YT158 细胞增殖示踪荧光探针(CFDA SE) Cell proliferation tracer fluorescence probe
BTN90408 即用型荧光定量PCR试剂盒 Instant Fluorescent qPCR Kit
SV1625 CLIP-Surface 488
SV0447 KpnI限制性内切酶 KpnI Restriction Endonuclease
WE0150 一步法逆转录实时荧光定量PCR试剂盒(高含量ROX校正染料) BaldStar TaqMan One Step RT-qPCR Kit(High ROX)
Klenow大片段库存关键词：Klenow大片段,百奥莱博,JN0070


·重组人UDP-葡萄糖-4-差向异构酶
编号：JN2033
英文名称：Recombinant Human UDP-Glucose 4-Epimerase
规格：10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成，目的基因编码的Met1-Ala348在N端含有His标签。

UDP-Glucose 4-Epimerase质量控制：＞95%(还原性SDS-PAGE)

UDP-Glucose 4-Epimerase制剂：液体

UDP-Glucose 4-Epimerase保存：收到货后请置于-20℃，可保存6个月，避免反复冻融。

关于GALE：
The enzyme UDP-Glucose 4-Epimerase is a homodimeric epimerase found in bacterial, plant and mammalian cells. UDP-Glucose 4-Epimerase performs the final step in the Leloir pathway of Galactose metabolism, it catalyzes two distinct but analogous reactions: the epimerization of UDP-Gglucose to UDP-Galactose and the epimerization of UDP-N-Acetylglucosamine to UDP-N-Acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.

北京百莱博科技有限公司专业生产工具酶产品，欢迎来电咨询选购Klenow大片段库存。