β-半乳糖苷酶ONPG定量检测试剂盒批发

特别声明：本产品及我公司所售其他产品均为科研类试剂产品，严禁用于药物、医疗及其他非科研用途。

北京百奥莱博主要从事生物试剂的生产和销*(代"售")，产品线涵盖β-半乳糖苷酶ONPG定量检测试剂盒批发在内的分子生物学试剂产品，还包括PCR及荧光定量PCR、核酸提取试剂盒类、样本核酸保护试剂、核酸提取、克隆与转化、蛋白纯化、蛋白检测、免疫组化、细胞凋亡与抗体等。

名称：β-半乳糖苷酶ONPG定量检测试剂盒批发
编号：JN0185
规格：200T
品牌：百奥莱博
产地：北京
  细菌中编码β-半乳糖苷酶(β-galactosidase)的LacZ基因常被用作报告基因。此酶最佳反应条件为中性pH，而哺乳动物细胞内源的半乳糖苷酶最适pH为酸性。在中性反应条件下哺乳动物内源性半乳糖苷酶对细菌半乳糖苷酶活性影响很小，因此细菌LacZ基因成为广泛使用的报告基因。ONPG是一种卓越的β-半乳糖苷酶底物。其完全可溶，并且在450nm。具有高的消光系数。与半乳糖苷酶反应后，产生一种黄色产物，用1M碳酸*终止后在可见光范围内进行检测。随试剂盒提供的细胞裂解液可以温和裂解贴壁或悬浮的哺乳动物细胞，用于beta半乳糖苷酶、荧光酶（luciferase）和CAT（chloramphenicol acetyltransferase）等报告基因系统的活性检测，目的蛋白活性比通过反复冻融法获得的要高。

试剂盒组成：

 

组份	规格
5× Reporter Lysis Buffer	30ml
2×ONPG Assay Buffer	10ml
1M Na2CO3	35ml

产品特点：
1、简单：整个kit由三个溶液组成：裂解液，显色液和终止液，依次加入即可，使用方便。
2、设备要求低：一般分光光度计或酶标仪（吸收波长：420nm）。
3、快速：整个检测过程可以在一个小时内完成。
4、灵敏度高，线性范围广。


β-半乳糖苷酶ONPG定量检测试剂盒批发正在火爆热销，除此之外，为您推荐下别热销产品：

·重组人S100-P蛋白
编号：JN2028
英文名称：Recombinant Human S100 Calcium Binding Protein P
规格：10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成，目的基因编码的Met1-Lys95在N端含有His标签。

Protein S100-P质量控制：＞95%(还原性SDS-PAGE)

Protein S100-P制剂：液体

Protein S100-P保存：收到货后请置于-20℃，可保存6个月，避免反复冻融。

关于Protein S100-P：
Protein S100-P (S100P) belongs to the S100 family of Calcium-Binding Proteins. S100P is 95 amino acids in length and it contains 2 EF-Hand Calcium-Binding Motifs. The EF-Hand Motif binds calcium, which likely alters molecular conformation. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and it is involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. In addition to binding Ca, S100P also binds Zn and Mg, and may play a role in the etiology of prostate cancer.


β-半乳糖苷酶ONPG定量检测试剂盒批发关键词：百奥莱博,β-半乳糖苷酶ONPG定量检测试剂盒,JN0185


·重组人肌醇单磷酸酶(IMPase2)(IMPA2)
编号：JN1748
英文名称：Recombinant Human Inositol Monophosphatase 2
规格：10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成，目的基因编码的Met1-Lys288在N端含有His标签。

IMPase2质量控制：＞95%(还原性SDS-PAGE)

IMPase2制剂：液体

IMPase2保存：收到货后请置于-20℃，可保存6个月，避免反复冻融。

关于IMPase2：
Inositol monophosphatase 2, also known as Inositol-1(or 4)-monophosphatase 2, Myo-inositol monophosphatase A2 and IMPA2, is an enzyme which belongs to the inositol monophosphatase family. IMPA2 catalyzes the dephosphoylration of inositol monophosphate with cofactor Magnesium and Inhibited by high Li+ and restricted Mg2+ concentrations. IMPA2 plays an important role in phosphatidylinositol signaling. IMPA2 can use the myo-inositol monophosphates, scylloinositol 1,4-diphosphate, glucose-1-phosphate, beta-glycerophosphate, and 2-AMP as substrates. IMPA2 is a pharmacological target for lithiu*(代"m") Li(+) action in brain, it is considered to have a role in schizophrenia and bipolar disorder.


β-半乳糖苷酶ONPG定量检测试剂盒批发关键词：百奥莱博,β-半乳糖苷酶ONPG定量检测试剂盒,JN0185


·重组人骨形成蛋白受体II(BMPR-II)
编号：JN0513
英文名称：Recombinant Human Bone Morphogenetic Protein Receptor Type IIA
规格：10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成，目的基因编码的Ser27-Ile151在C端含有His标签。

BMPR-II质量控制：＞95%(还原性SDS-PAGE)

BMPR-II制剂：冻干品

BMPR-II保存：
冻干蛋白置于-20℃以下可长期保存，室温条件下可稳定保存3周。
复溶蛋白溶液可在4～7℃保存2～7天，可分装后置于-20℃保存三个月。

BMPR-II复溶：
打开试剂管前请先离心。
复溶浓度推荐大于100μg/ml。
冻干蛋白请溶于ddH2O。
复溶后，请根据用量分装冻存，避免反复冻融。

关于BMPR-II：
Bone Morphogenetic Protein Receptor II (BMPR-II) is a Type II Serine/Threonine Kinase that mediates cellular responses to BMPs. BMPR-II is characterized by lacking of a GS domain, and presence of a C-terminal extension typical of type II receptors. BMPRII binds BMP2, BMP4 and BMP7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor, including BMPR-IA/Brk1, BMPR-IB, and ActR-I. BMPR-II plays a key role in cell growth. Defects in BMPR-II have been linked to primary pulmonary hypertension. Human and mouse BMPR-II are highly conserved and share 97 % amino acid sequence identity.

我公司生产供应销*(代"售")的蛋白质研究产品正全系列现货促销，满分期待您的垂询选购β-半乳糖苷酶ONPG定量检测试剂盒批发。