- ¥780 - 2200
- 康朗生物
- kl-2499R
- 中国/美国/德国
- 2025年07月04日
- WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500
- Rabbit
- Human, Mouse, Rat, Dog,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海康朗生物科技有限公司
- 库存：
大量
- 目录编号：
kl-2499R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
12个月
- 抗体英文名：
CD69 antibody
- 抗体名：
活化诱导分子CD69抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog,
- 免疫原：
KLH conjugated synthetic peptide derived from human CD69:101-199/199 <Extracellular>
- 亚型：
IgG
- 形态：
冻干粉或液体
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
50ul 100ul 200ul
| 中文名称 | 活化诱导分子CD69抗体 |
| 别 名 | CLEC2C; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; EA1; BL-AC/P26;C-type lectin domain family 2 member C. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 干细胞 糖尿病 细胞表面分子 自然杀伤细胞 淋巴细胞 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 22kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD69:101-199/199 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011] Function: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. Subunit: Homodimer; disulfide-linked. Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. Post-translational modifications: Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. Similarity: Contains 1 C-type lectin domain. SWISS: Q07108 Gene ID: 969 Database links: Entrez Gene: 969 Human Entrez Gene: 12515 Mouse Entrez Gene: 29187 Rat Omim: 107273 Human SwissProt: Q07108 Human SwissProt: P37217 Mouse Unigene: 208854 Human Unigene: 74745 Mouse Unigene: 23779 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD69属于C-型凝集素受体家族,也是NK细胞信号传导基因复合体家族的一员,是一种Ⅱ型跨膜糖蛋白. |
| 产品图片 |  Sample: U937 Cell Lysate at 30 ug Primary: Anti- CD69 (bs-2499R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 34 kD  Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)) . Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody (bs-2499R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions. |
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文献和实验CD69 分子 CD69 常用单克隆抗体或代号： Leu23;(VEA,AIM) 主要表达细胞： Ta,Ba,Mac,NK,Pt [NK] 分子质量(kDa)和结构： 34,28(同源二聚体,C- lectin SF) 功 能： 活化诱导分子,参与信号转导,Eo凋亡,参与TCRd g 溶细胞功能 CD69 Aka activation inducer molecule (AIM), early activation antigen (EA
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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