CD86抗体

价格¥780 - 2200

品牌LMAI Bio
货号LM-1035R
产地进口/国产
更新日期2025年07月15日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
宿主Rabbit
适应物种Human, Mouse, Rat, Dog, Pig, Cow, Sheep,

上海联迈生物工程有限公司

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详细信息
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技术资料

供应商：
上海联迈生物工程有限公司
库存：
大量
目录编号：
LM-1035R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
1年
抗体英文名：
CD86
抗体名：
CD86抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Sheep,
免疫原：
KLH conjugated synthetic peptide derived from the middle of human CD86 (140-175aa):140-175/313 <Extracellular>
亚型：
IgG
形态：
Lyophilized or Liquid
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
浓度：
1mg/ml
保存条件：
Store at -20 °C
规格：
50ul 100ul 200ul

CD86抗体

英文名称	CD86
中文名称	CD86抗体
别名	CD28LG2; FUN1; LAB72; Activation B7 2 antigen; Activation B7-2 antigen 3; CD86_HUMAN; Activation B7-2 antigen; Activation B72 antigen; B lymphocyte activation antigen B7 2; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7 2 antigen; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD 86; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen; CD86 molecule; CLS1; CTLA 4 counter receptor B7 2; CTLA 4 counter receptor B7.2; CTLA-4 counter-receptor B7.2 2, 3; CTLA4 counter receptor B72; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; TS/A-2.
	Specific References (10) \| bs-1035R has been referenced in 10 publications. [IF=0.00] Evans, Elizabeth E., et al. "Antibody blockade of semaphorin 4D promotes immune infiltration into tumor and enhances response to other immunomodulatory therapies." Cancer Immunology Research (2015): canimm-0171. IHC-P ; Mouse. PubMed:25614511 [IF=0.90] Zhou, Xi-Jin, et al. "Vasoactive Intestinal Peptide Promotes Immune Escape of MKN45 Cells by Inhibiting Antigen-Presenting Molecules of Dendritic Cells In Vitro." International Journal of Peptide Research and Therapeutics: 1-13. other ; PubMed:not posted yet [IF=2.96] Xie, Juan, et al. "PICK1 confers anti-inflammatory effects in acute liver injury via suppressing M1 macrophage polarization." Biochimie (2016). WB ; Mouse. PubMed:27157267 [IF=1.55] Chen, Quan, et al. "Prevention of ultraviolet radiation‑induced immunosuppression by sunscreen in Candida albicans‑induced delayed‑type hypersensitivity." Molecular Medicine Reports (2016). WB ; Mouse. PubMed:27175551 [IF=5.76] Das, Subhamoy, et al. "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." Advanced Healthcare Materials (2016). IHC-P ; Mouse. PubMed:27385307 [IF=2.16] Ju, Huanyu, et al. "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." BMC immunology 17.1 (2016): 31. IHC-P ; Mouse. PubMed:27669687 [IF=1.58] Liang, Zhuoping, et al. "Role of dendritic cells in consistency between upper and lower airway inflammatory responses: animal experimental studies." Int J Clin Exp Pathol 9.10 (2016): 10093-10104. IHC-P ; Rat. PubMed:0 [IF=1.48] Zhang, Suxin, et al. "Variation and significance of secretory immunoglobulin A, interleukin 6 and dendritic cells in oral cancer." Oncology Letters. IHC-P ; Human. PubMed:28454394 [IF=3.15] Chen, Xionglin, et al. "Peptide-modified chitosan hydrogels promote skin wound healing by enhancing wound angiogenesis and inhibiting inflammation." Am J Transl Res 9.5 (2017): 2352-2362. IHC-P ; Mouse. PubMed:28559985 [IF=5.19] Han, Sheng, et al. "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 83. WB ; Human. PubMed:28641579
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	免疫学微生物学干细胞细胞表面分子糖蛋白淋巴细胞 t-淋巴细胞 b-淋巴细胞
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Pig, Cow, Sheep,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	31kDa
细胞定位	细胞膜
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from the middle of human CD86 (140-175aa):140-175/313 <Extracellular>
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]. Function: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. Subunit: Homodimer. Interacts with MARCH8. Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed by activated B-lymphocytes and monocytes. Post-translational modifications: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: O35531 Gene ID: 942 Database links: Entrez Gene: 942 Human Entrez Gene: 56822 Rat Omim: 601020 Human SwissProt: P42081 Human Unigene: 171182 Human Unigene: 229570 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD86是一个很重要要的辅助刺激分子，CD86作为CD28的辅助刺激分子，提供 T 细胞活化的辅助信号,它们之间的结合和随后介导的信号转导是 T 细胞和 APC 相互协作的重要分子基础。
产品图片	Sample: Lymph node(Mouse)Cell Lysate at 40 ug Primary: Anti-CD86 (bs-1035R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD Sample: Brain(Rat) lysates at 30ug; Heart(Rat) lysates, 30ug; Primary: Anti-CD86/B7-2 (bs-1035R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 34kD Observed band size : 34kD Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated(bs-1035R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated(bs-1035R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: BV-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CD86) Polyclonal Antibody, Unconjugated (bs-1035R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Blank control: U937(blue). Primary Antibody: Rabbit Anti-CD86 antibody(bs-1035R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (bs-1035R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.