2,4,6-trimethyl-1,3,5,2,4,6-tr

Synthesis of an Esterase-Sensitive Cyclic Prodrug of a Model Hexapeptide Having Enhanced Membrane Permeability and Enzymatic Stability Using a 3-(2-Hy

by taking advantage of a unique “trimethyl lock”-facilitated lactonization system (Fig. 1 ). Substituted phenol propionic acid derivatives such as 2 , upon unmasking of the hydroxyl group, undergo a facile spontaneous intramolecular cyclization to release

真菌DNA提取(Lazo Lab)

at a lower viscosity.CTAB extraction buffer: O.1M Tris, pH 7.5, 1% CTAB (mixed hexadecyl trimethyl ammonium bromide), 0.7M NaCl, 10mM EDTA, 1% 2-mercaptoethanol. Add proteinase K to a final concentration of 0.3 mg/ml prior to use. Less prot K

真菌DNA提取

to keep the samples at a lower viscosity.CTAB extraction buffer: O.1M Tris, pH 7.5, 1% CTAB (mixed hexadecyl trimethyl ammonium bromide), 0.7M NaCl, 10mM EDTA, 1% 2-mercaptoethanol. Add proteinase K to a final concentration of 0.3 mg/ml prior to use. Less prot K