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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™
- 浓度:
5 µL/Test
- 应用范围:
见产品说明书
- 宿主:
Mouse
- 库存:
需确认
- 适应物种:
见产品说明书
- 保质期:
见产品说明书
- 标记物:
见产品说明书
- 是否单克隆:
其他
- 保存条件:
4° C
- 亚型:
IgG2b
- 规格:
100 Tests
货号 12-4732-42
100 Tests产品描述
Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This mouse IgG2b isotype control has been reported for use in flow cytometric analysis.
Applications Tested: This Mouse IgG2b K Isotype Control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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Roche试剂,部分常用现货,周三定货,2-3周到货。
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SIGMA试剂,周一,五定货,3周左右到货。
THERMO试剂,部分现货,周三定货,2-3周到货。
ABCAM试剂,国内现货三天左右,期货四周左右。
Peprotech试剂,部分常用现货,周三定货,2-3周到货。
罗氏原料,请来电来邮咨询。
OXOID,BD培养基础货期八周左右,部分现货。
MCE激动剂,ACROS、ALFA、阿拉丁等生化试剂现货定购。
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
D Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A D8 IgG2b, 09821D Eryth: Ter-119 IgG2b, 09081A Sca-1 PE-IgG2a (E31-161.7) 01835B PE-IgG2a Isotype control, 11025
样本 同型对照管:纯化的同型对照+PE标记的抗Mouse IgG1+样本 如果没有找到适合的同型对照,在保持来源相同、荧光标记相同、免疫球蛋白类别相同条件下,那么优先选择的顺序应该是亚链不同--亚型不同--相同类别。比如,某种的抗体的来源是Mouse IgG2а,κ。如果在同型对照表里面找到不到完全相同的同型对照,那么优先选择相同荧光标记的Mouse IgG2а为同型对照。如果也没有找到Mouse IgG2а,那么优先选择相同荧光标记的Mouse IgG2b
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