醇脱氢酶-11531034103

Alcohol Dehydrogenase 醇脱氢酶-11531034103
from yeast, suspension
Dehydrogenase that catalyzes the interconversion of alcohols to the corre-
sponding aldehydes.
Application
Use Alcohol Dehydrogenase in diagnostic tests for the determination of
alcohol or aldehyde (formate).
Benefits
Apply this ready-to-use enzyme directly in your diagnostic test. ■
Rely on the proven diagnostic quality of this product. ■
EC 1.1.1.1
Properties
Nomenclature: Alcohol:NAD + oxidoreductase
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10 -2 mol/l
NAD: 7.4 x 10 -5 mol/l
Acetaldehyde: 7.8 x 10 -4 mol/l
NADH: 1.1 x 10 -5 mol/l
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10 -2 mol/l
NAD: 6.1 x 10 -4 mol/l
Acetaldehyde: 6.7 x 10 -4 mol/l
NADH: 1.8 x 10 -5 mol/l
Inhibitors:
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate,
iodoacetic acid, N-substituted maleinimides, Hg 2+ , Ag + and Cu 2+ .
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine,
cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H 2 O 2 and aerial oxygen inactivate by oxidation of essential
groups.
pH optimum: 9.0 (see figure)
Temperature dependence: See figure
pH stability: 6.0-8.0 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropa-
nol and secondary butanol are slowly oxidized, while higher secondary and
tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse
reaction. The enzyme does not react with NADP.
Remark: Alcohol dehydrogenase tends to show turbidity in solution at +37°C
storage.
Modified Alcohol dehydrogenase shows no turbidity for at least 4 weeks in
solution at +37°C.
Specification
Appearance: White to yellow-brown crystalline suspension in ammonium
sulfate solution, 3.2 mol/l, pH approximately 6.5
pH value: 6.0-7.0
Activity: 9,000 to 18,000 U/ml
Specific activity: ≥300 U/mg (protein)
Protein (Biuret): 30±3 mg/ml lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase: ≤0.01
Stability: At +2 to +8°C within specification range for 9 months. Store under
nitrogen.