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- 11452541103
- 2025年08月25日
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- 详细信息
- 技术资料
- 供应商：
嵘崴达
- 库存：
需确认
- 英文名：
Alcohol Dehydrogenase, Yeast
- 保质期：
见产品外包装
- 保存条件：
见说明书
Alcohol Dehydrogenase 醇脱氢酶-11452541103
from yeast, lyophilizate
Dehydrogenase that catalyzes the interconversion of alcohols to the corre-
sponding aldehydes.
Application
Use Alcohol Dehydrogenase in diagnostic tests for the determination of
alcohol or aldehyde (formate).
Benefits
Rely on the proven diagnostic quality of this product.
EC 1.1.1.1
Properties
Nomenclature: Alcohol:NAD + oxidoreductase
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10 -2 mol/l
NAD: 7.4 x 10 -5 mol/l
Acetaldehyde: 7.8 x 10 -4 mol/l
NADH: 1.1 x 10 -5 mol/l
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10 -2 mol/l
NAD: 6.1 x 10 -4 mol/l
Acetaldehyde: 6.7 x 10 -4 mol/l
NADH: 1.8 x 10 -5 mol/l
Inhibitors:
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate,
iodoacetic acid, N-substituted maleinimides, Hg 2+ , Ag + and Cu 2+ .
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine,
cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H 2 O 2 and aerial oxygen inactivate by oxidation of essential
groups.
pH optimum: 9.0 (see figure)
Temperature dependence: See figure
pH stability: 6.0-8.0 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropa-
nol and secondary butanol are slowly oxidized, while higher secondary and
tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse
reaction. The enzyme does not react with NADP.
Remarks:Alcohol dehydrogenase tends to show turbidity in solution at +37°C
storage. Modified Alcohol dehydrogenase shows no turbidity for at least 4
weeks in solution at +37°C.
Specification
Appearance: White lyophilizate (50 mg lyophilizate contain approximately 30
mg enzyme protein,15 mg sucrose, 5 mg phosphate)
pH value (c=50 mg/ml in water): 7.0-8.0
Activity (+25°C, ethanol): No limit
Specific activity: ≥400 U/mg (protein)
Protein (Biuret): ≥0.5 mg/mg lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase ≤0.01
Stability: At -15 to -25°C within specification range for 12 months. Store dry.
from yeast, lyophilizate
Dehydrogenase that catalyzes the interconversion of alcohols to the corre-
sponding aldehydes.
Application
Use Alcohol Dehydrogenase in diagnostic tests for the determination of
alcohol or aldehyde (formate).
Benefits
Rely on the proven diagnostic quality of this product.
EC 1.1.1.1
Properties
Nomenclature: Alcohol:NAD + oxidoreductase
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10 -2 mol/l
NAD: 7.4 x 10 -5 mol/l
Acetaldehyde: 7.8 x 10 -4 mol/l
NADH: 1.1 x 10 -5 mol/l
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10 -2 mol/l
NAD: 6.1 x 10 -4 mol/l
Acetaldehyde: 6.7 x 10 -4 mol/l
NADH: 1.8 x 10 -5 mol/l
Inhibitors:
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate,
iodoacetic acid, N-substituted maleinimides, Hg 2+ , Ag + and Cu 2+ .
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine,
cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H 2 O 2 and aerial oxygen inactivate by oxidation of essential
groups.
pH optimum: 9.0 (see figure)
Temperature dependence: See figure
pH stability: 6.0-8.0 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropa-
nol and secondary butanol are slowly oxidized, while higher secondary and
tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse
reaction. The enzyme does not react with NADP.
Remarks:Alcohol dehydrogenase tends to show turbidity in solution at +37°C
storage. Modified Alcohol dehydrogenase shows no turbidity for at least 4
weeks in solution at +37°C.
Specification
Appearance: White lyophilizate (50 mg lyophilizate contain approximately 30
mg enzyme protein,15 mg sucrose, 5 mg phosphate)
pH value (c=50 mg/ml in water): 7.0-8.0
Activity (+25°C, ethanol): No limit
Specific activity: ≥400 U/mg (protein)
Protein (Biuret): ≥0.5 mg/mg lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase ≤0.01
Stability: At -15 to -25°C within specification range for 12 months. Store dry.
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醇脱氢酶-11452541103
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