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- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Titan One Tube RT-PCR System
- 保质期：
见产品外包装
- 供应商：
嵘崴达
- 保存条件：
−20°C
- 库存：
需确认
- 规格：
100 reactions
Titan One Tube RT-PCR 系统
Titan One Tube RT-PCR System
General description
The Titan™ One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:
• Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
• Delivers increased sensitivity due to the high efficiency of all three enzymes.
• Is faster than conventional two-step RT-PCR.
Specificity
反应特异性很大程度上取决于引物设计和DNA污染情况。
为了排除DNA靶向检测中的人为干扰，反应中应引入适当的阳性对照与阴性对照。
热灭活：94℃下处理2分钟
逆转录酶AMV
Application
Titan™ One Tube RT-PCR System has been used:
• to amplify the regions containing the miRNA target (miRT) inserts/response elements in viral RNA
• for determining the presence or absence of RNA templates
• in cloning of rare messages without the need to construct cDNA libraries
• for quantifying the levels of gene expression
Features and Benefits
Titan™ One Tube RT-PCR System:
• High Fidelity: Expand™ High Fidelity enzyme blend with proofreading activity reduces the error rate in polymerase chain reaction (PCR)
• offers a threefold higher fidelity in comparison to Taq DNA polymerase
• delivers increased sensitivity due to the high efficiency of all three enzymes
• faster than conventional two-step reverse transcriptase (RT)-PCR
• allows the amplification of full-length cDNA up to 6 kb by RT-PCR due to the use of the Expand™ PCR System
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
could be done in a single tube using Titan One Tube RT-PCR System (Boehringer Mannheim) or One Step RNA PCR Kit (Takara). Both gave satisfactory results. Total RNA was extracted from organs, tissues, or tissue culture cells as described above. Poly(A)+ RNA was purified
Methods for the Detection of D-Amino-Acid Oxidase
and PCR were carried out separately. However, these two steps could be done in a single tube using Titan One Tube RT-PCR System (Boehringer Mannheim) or One Step RNA PCR Kit (Takara). Both gave satisfactory results. Total RNA was extracted from organs
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