11014714001-Not I

Not I

from Nocardia otitidis-caviarum

别名:
restriction enzyme

一般描述

Not I recognizes the sequence GC?GG*C*CG°C and generates fragments with 5′-cohesive termini.
Not I belongs to the class of "rare-cutter" enzymes. It is one of the two known enzymes that recognize an octameric sequence comprised solely of G and C residues.

Contents:

• Not I
• SuRE/Cut Buffer H (10x)

特异性

Recognition sites: GCGG*C*CG °C
GCGG*C*CG °C
Restriction site: GC↓GG*C*CG °C
GC↓GG*C*CG °C
Heat inactivation: Not I can be heat inactivated by incubation at 65 °C for 15 minutes (up to 100 U/μg DNA).

质量

Absence of nonspecific endonuclease activities
1 μg Ad2 DNA is incubated for 16 hours in 50 μl SuRE/Cut Buffer H with an excess of Not I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.

Absence of exonuclease activity
Approximately 5 μg [³H] labeled calf thymus DNA are incubated with 3 μl Not I for 4 hours at +37°C in a total volume of 100 μl 50 mM Tris-HCl, 10 mM MgCl₂, 1 mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.

上海嵘崴达实业有限公司，专业经营生命科学领域的科研试剂、仪器和实验室消耗品，

致力于向国内生命科学研究人员提供合格产品和完善服务。

主要经营的试剂品牌：
Roche试剂，部分常用现货，周三定货，2-3周到货。
GE试剂，耗材，部分常用现货，周二，周五定货，3周左右。
SIGMA试剂，周一，五定货，3周左右到货。
THERMO试剂，部分现货，周三定货，2-3周到货。
ABCAM试剂，国内现货三天左右，期货四周左右。
Peprotech试剂，部分常用现货，周三定货，2-3周到货。

罗氏原料，请来电来邮咨询。

OXOID，BD培养基货期八周左右，部分现货。
MCE激动剂，ACROS、ALFA、阿拉丁等生化试剂现货定购。