木瓜蛋白醇

木瓜蛋白醇

Papain

from Carica papaya

General description

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Application

Papain has been used in the digestion of various brain cells.

木瓜蛋白酶可用于蛋白质的完全蛋白水解切割、天然免疫球蛋白的有限水解以及组织解离（与胶原酶、酯酶和胰蛋白酶一起使用）。该酶可溶解内在膜蛋白并水解纯蛋白多糖生成糖肽。添加半胱氨酸（约0.5% [w/v]）对酶活性至关重要。

Biochem/physiol Actions

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Physical form

悬浮液，结晶，无菌

Preparation Note

工作浓度：0.05 至 0.5 mg/ml

Storage and Stability

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)
 

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