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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Recombinant PPase
- 保质期:
一年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
-20℃或更低温度保存
- 规格:
100IU
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| LM7073-100IU | Recombinant PPase | 100IU | 927.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| - | - | - | E. coli | - | - | - |
| About this Protein | |
| Name | Recombinant PPase (Recombinant PreScission Protease; rPP);重组前切割蛋白酶 |
| Synonyms | N/A |
| Purity | N/A |
| Biological Activity | N/A |
| Physical Appearance | N/A |
| Formulation | N/A |
| Endotoxin | N/A |
| Reconstitution | During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments.Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease. |
| Category | Enzymes |
| Background | PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease . The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues . Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well. |
| Amino Acid Sequence | N/A |
| 产品编号 | 产品名称 | 包装 |
| LM7073-100IU | Recombinant PPase | 100IU |
| LM7073-250IU | Recombinant PPase | 250IU |
| LM7073-5000IU | Recombinant PPase | 5000IU |
| - | 说明书 | 1份 |
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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