- ¥1205
- 联迈生物
- LM7068-1000IU
- 中国/上海
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Recombinant Tobacco EV
- 保质期：
一年
- 供应商：
上海联迈生物工程有限公司
- 保存条件：
-20℃或更低温度保存
- 规格：
1000IU
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| LM7068-1000IU | Recombinant Tobacco EV | 1000IU | 1205.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Tobacco | 1502321 | LM04517 | E. coli | － | － | － |
| About this Protein | |
| Name | Recombinant Tobacco EV (Recombinant Tobacco Etch Virus Protease; rTEV)；重组烟草蚀纹病毒蛋白酶 |
| Synonyms | polyprotein; 6K2 protein; NIa－VPg protein; NIb protein; coat protein; P1 protein; HC－Pro protein; P3 protein; 6K1 protein; CI protein; NIa－Pro protein |
| Purity | >90% by SDS-PAGE analysis. |
| Biological Activity | N/A |
| Physical Appearance | Clear colorless liquid. |
| Formulation | A 0.2μm filtered solution in 25mM Tris－HCl, pH8.0, 75mM NaCl, 5mM EDTA, 10mM GSH, with 50% Glycerol. |
| Endotoxin | N/A |
| Reconstitution | A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat－labile, then 4℃ incubations are recommended. Reactions at 4℃ will require longerincubation times and/or more rTEV. |
| Category | Enzymes |
| Background | TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a (NIa) protein. It is consists of 241a.a. with the molecular weight of 27kDa. TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X', and cleaves between Q (or S)/X'. In this form X and X' stand for any of the amino acid residues, except that X' cannot be P. The optimal cleavage site is ENLYFQ/G. As having the absolute specificity and wildly using conditions like broad pH range and ionic strength, the TEV protease became more versatile than EK, thrombin and other protease used in biochemical applications, especially recombinant protein production. The optimal temperature for cleavage is 30℃; however, the enzyme can be used at temperatures as low as 4℃. Following digestion, TEV Protease can be removed from the reaction via the GST tag sequence by affinity chromatography. |
| Amino Acid Sequence | N/A |
| 产品编号 | 产品名称 | 包装 |
| LM7068-300IU | Recombinant Tobacco EV | 300IU |
| LM7068-1000IU | Recombinant Tobacco EV | 1000IU |
| LM7068-10000IU | Recombinant Tobacco EV | 10000IU |
| - | 说明书 | 1份 |
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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