RIP-seq(RNA immunoprecipitation and deep sequencing)是RNA免疫沉淀与高通量测序结合的技术,它通过免疫沉淀目标蛋白来捕获蛋白体内结合的RNA。将捕获的RNA进行高通量测序,可获得RNA结合蛋白(RBP)在体内与众多RNA靶标的结合模式,并对其结合强度进行精确定量,最终通过分析目的蛋白在体内结合RNA的动态变化,阐述出目的蛋白对基因表达调控的分子机制。
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Shi Y, Fan S, Wu M, et al. YTHDF1 links hypoxia adaptation and non-small cell lung cancer progression[J]. Nature Communications. (IF:12.121)
Jun He, Fazhao Li, Yan Zhou, Xuyang Hou, Sushun Liu, Xinchun Li, Yawei Zhang,Xiaoqian Jing, Leping Yang. LncRNA XLOC_006390 promotes pancreatic carcinogenesis and glutamate metabolism by stabilizing c-Myc.J He et al. Cancer Lett (2020) (IF:7.360)
Xinxin Zhang, Yilong Yao, Jinghua Han, Yalan Yang, Yun Chen, Zhonglin Tang, Fei Gao,Longitudinal epitranscriptome profiling reveals the crucial role of N6-methyladenosine methylation in porcine prenatal skeletal muscle development,Journal of Genetics and Genomics,2020, (IF:5.065)