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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现货
- 英文名:
Cell Proliferation Kit II (XTT)
- 供应商:
上海善然生物科技有限公司
- 保存条件:
低温
Features and Benefits• Safe and easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
• Accurate: The absorbance obtained strongly correlates to the cell number.
• Sensitive: Detect low cell numbers.
• Fast: Process a large number of samples using a multi-well ELISA reader.
General description下载特定产品批次和规格的CofA、SDS和IFU。 The Cell Proliferation Kit II (XTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.
Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells, and the complete assay, from microculture to data analysis by an ELISA reader, is performed in the same microplate.
More recently, the tetrazolium salt XTT was described. In contrast to MTT, the cleavage product of XTT is soluble in water; therefore, a solubilization step is not required. The tetrazolium salt XTT is cleaved to formazan by a complex cellular mechanism. This bioreduction occurs in viable cells only, and is related to NAD(P)H production through glycolysis. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.Other Notes仅用于生命科学研究。不可用于诊断。包装1 kit containing 2 components.
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文献和实验相关专题 材料与方法 1. 主要试剂:MTT、XTT、PMS、DMSO (Sigma公司产品),RPMI 1640(Gibco公司),氟尿嘧啶(上海旭东海普药业有限公司),注射用盐酸阿霉素(深圳万乐药业有限公司),注射用丝裂霉素C(协和发酵工业株式会社),新生小牛血清(杭州四季青公司提供)。 2. 细胞与细胞培养 :人肝癌细胞系 BEL-7402置含10%新生小牛血清的RPMI 1640培养
[基本原理] XTT比色法有Scudiero等首次采用,用于检测细胞增殖。XTT是异种与MTT类似的四唑氮衍生物,可被活细胞线粒体脱氢酶还原成水溶性的棕色甲肷产物,当XTT与电子偶合剂共同使用时,甲肷的生成量与细胞的增殖程度呈正相关。 [试剂及材料] (1)XTT:用60℃预热培养液配制成6.6mmol/L,过滤除菌,现配现用。 (2)吩嗪二甲酯硫酸盐(PMS):用PBS配制成220mmol/L,4℃避光保存20天。 (3) XTT/PMS:XTT与PMS
XTT 检测法 用 XTT 代替 MTT 可省去溶解还原产物结晶的步骤, XTT 可以被活细胞中的代谢酶还原成黄色水溶性的代谢产物( formazan )。代谢产物在 OD450 处有吸收峰。
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