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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Recombinant Rhesus Macaque Flt-3 Ligand
- 保质期:
一年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
-20℃或更低温度保存
- 规格:
10μg
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| LM5885-10μg | Recombinant Rhesus Macaque Flt-3 Ligand | 10μg | 1152.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Rhesus Macaque | 719239 | LM9Z6V7 | E. coli | 159aa | 18kDa | - |
| About this Antibody | |
| Name | Recombinant Rhesus Macaque Flt-3 Ligand (Recombinant Rhesus Macaque Fms-related Tyrosine Kinase 3 Ligand; rRhFlt-3L);重组恒河猴Fms相关酪氨酸激酶3配体 |
| Synonyms | Fms-related tyrosine kinase 3 ligand; FLT3LG |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human Aml5 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×106IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered solution in PBS, pH7.4. |
| Endotoxin | Less than 1EU/μg of rRhFlt-3L as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Flt-3 ligand (FL) is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3. Flt-3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additionally, class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt-3 ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors. |
| Amino Acid Sequence | TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVPSNLQD EELCGALWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQHP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPRSPGALEA TALTAPQRP |
| 产品编号 | 产品名称 | 包装 |
| LM5885-2μg | Recombinant Rhesus Macaque Flt-3 Ligand | 2μg |
| LM5885-10μg | Recombinant Rhesus Macaque Flt-3 Ligand | 10μg |
| LM5885-100μg | Recombinant Rhesus Macaque Flt-3 Ligand | 100μg |
| LM5885-1mg | Recombinant Rhesus Macaque Flt-3 Ligand | 1mg |
| - | 说明书 | 1份 |
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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文献和实验Adjuvant Activity of Cytokines
by encapsulation into liposomes and the use of cytokine expression vectors co-administered with DNA vaccines. Although a number of cytokines including IFN-α, IFN-γ, IL-2, IL-12, IL-15, IL-18, IL-21, GM-CSF, and Flt-3 ligand have been shown to potentiate the immune
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
1 cells are used to generate T-lineage cells, and OP9 cells are used to generate B-lineage and myelo-erythroid cells. OP9 medium Phosphate-buffered saline (PBS; Hyclone SH30256) Recombinant human Flt-3/Flk-2 ligand (R&D Systems 308-FK
【求助】新手 请问western-blot测NF-KB、FLT3是用总蛋白提取试剂盒吗
-3, extracellular region; 200 µg /Anti-Flt-3, cytoplasmic domain; 200 µg /Flt-3 Ligand, recombinant human; 10 µg 。不知道用那一种好!可惜我是搞临床的,免疫和分子生物学很差,知道不多,问了该公司技术支持,也没说出什么,建议查参考文献。东查西查也没有弄清?急盼各位高手给答案。万分感谢! lwjssry 看了你描述的三种抗体,推测flt3是一种膜受体吧,不知猜得对不对?
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