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Recombinant Human Flt-3 Ligand

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  • ¥464
  • 联迈生物
  • LM5345-2μg
  • 中国/上海
  • 2025年07月16日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      大量

    • 英文名

      Recombinant Human Flt-3 Ligand

    • 保质期

      一年

    • 供应商

      上海联迈生物工程有限公司

    • 保存条件

      -20℃或更低温度保存

    • 规格

      2μg

    Recombinant Human Flt-3 Ligand
    产品编号 产品名称 产品包装 产品价格
    LM5345-2μg Recombinant Human Flt-3 Ligand 2μg 464.00
     
    Species Gene ID Accession Source Length MW Tag
    Human 2323 LM49771 E. coli 155aa 17.6kDa
     
    About this protein
    Name Recombinant Human Flt-3 Ligand (Recombinant Human Fms-related Tyrosine Kinase 3 Ligand; rHuFlt3-Ligand);重组人Fms相关酪氨酸激酶3配体
    Synonyms FLT3LG; FL; Flt3 ligand; FLT3L; Flt3L; fms-related tyrosine kinase 3 ligand; SL cytokine
    Purity >97% by SDS-PAGE and HPLC analyses.
    Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×106IU/mg.
    Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
    Formulation Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.
    Endotoxin Less than 1EU/μg of rHuFlt3-Ligand as determined by LAL method.
    Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
    Category Cytokine
    Background Flt3-ligand (FL) is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt3. Flt3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additionally, class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt3-ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. An alternatively-spliced FL mRNA, encoding a soluble form of the human FL, has also been identified. FL is widely expressed in various human and mouse tissues. At the amino acid sequence level, human and mouse FL are approximately 72% identical and the two proteins exhibit cross-species activity. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.
    Amino Acid Sequence TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
    包装清单:
    产品编号 产品名称 包装
    LM5345-2μg Recombinant Human Flt-3 Ligand 2μg
    LM5345-10μg Recombinant Human Flt-3 Ligand 10μg
    LM5345-100μg Recombinant Human Flt-3 Ligand 100μg
    LM5345-1mg Recombinant Human Flt-3 Ligand 1mg
    - 说明书 1
    保存条件:
           -20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
    注意事项:
           由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
           本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
           为了您的安全和健康,请穿实验服并戴一次性手套操作。


     

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