- ¥2480
- Vectorlabs
- 美国
- SK-5100
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
参照说明书
- 保质期：
参照说明书
- 英文名：
VECTOR RED SUBSTRATE KIT
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
1 Kit
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文献和实验the secondary antibody at a 1/400 dilution. Apply 150µl and incubate 30 min., RT in a humid chamber. Blot off the antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of ABC-Vector Red complex from the Vector ABC kit (Catalog No. AK
to the growing cells at a final concentration of 20 μg/ml. Construction of the pSC101BADγβαA[hygro] vector The pSC101BADγβαA[hygro] plasmid was based on the pSC101BADγβαA[tet] vector (GeneBridges) and was constructed by standard Red/ET
in every 2-3 minutes. Important: Monitor the pH of the Gel/Binding buffer mixture after the gel completely dissolved. DNA yield will significantly decreased when pH > 8.0. If the color of the mixture become orange or red, Add 5 ul of 5M sodium acetate, pH 5.2
