研选同类产品更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 英文名：
Hito Golgi-Cox OptimStain Kit
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
1Kit
Hitobiotec Golgi-Cox OptimStain™ Prekit 高尔基染色试剂盒
关键词
高尔基染色试剂盒，高尔基，Golgi Staining,Hitobiotec Corp.；Golgi-Cox impregnation 浸染法；neuron 神经元（神经细胞）；glia 神经胶质（胶质细胞）
产品描述
Golgi-Cox 染色或Golgi-Cox 浸染法（Golgi-Cox impregnation）公认为是用来研究神经元和神经胶质细胞形态的最有效和美观的通用方法之一。近些年高尔基体染色Golgi-Cox 染色法依然是用来检查树突分枝模式和树突棘的优选方法，因其能对大脑某特定区域随机挑取的少数片段内的树突树（dendritic arbour）进行分离和鉴定。
高尔基染色技术不仅只用于解剖研究，而且广泛用于行为-形态关系研究。一些研究中Golgi-Cox法曾用来鉴定和研究心脏自主神经支配，结果显示Golgi-Cox法是一种非常有效的技术来鉴定和研究外周血组织自主神经支配的形态学特征。然而，Golgi-Cox染色法很费时，需要使用振动切片机，细胞染色率通常比较低，结果常常不可靠。
Hitobiotec Corp.公司生产的高尔基体染色试剂盒（Hito Golgi-Cox OptimStain™ Kit）针对以上问题提供了一种简单的解决方案。基于Glaser and Van der Loos描述的方法，本试剂盒对Golgi-Cox 技术做出了显著改善。此试剂盒步骤简单且操作时间明显降低。科研人员可使用-19ºC保存的冷冻切片机（cryostat）。不仅提供稳定和改善的染色质量，而且只要适当操作几乎无过度染色。标准试剂盒可用于50个小鼠脑切片染色。
如果没有冷冻切片机（cryostat），组织可用薄片切片机（microtome）制备成石蜡切片。
Hitobiotec Corp.公司生产的高尔基体染色试剂盒（Hito Golgi-Cox OptimStain™ Kit）经测试广泛用于不同动物的脑、脊髓和心脏组织，以及高灵敏度显示神经元和神经胶质细胞的形态学细节。
试剂盒组分
染色结果示例：
注意：
引用文献：
引用文献：
1.BH Kan,etal.Acupuncture improves dendritic structure and spatial learning and memory ability of Alzheimer's disease mice.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108219/
2.ZhiqiangWang,etal.Chronic cerebral hypoperfusion induces long-lasting cognitive deficits accompanied by long-term hippocampal silent
synapses increase in rats.
文献链接：https://doi.org/10.1016/j.bbr.2015.12.047
3.J Yuan, H Huang,etal.MicroRNA-132 interact with p250GAP/Cdc42 pathway in the hippocampal neuronal culture model of acquired epilepsy and associated with epileptogenesis process.
文献链接：https://www.hindawi.com/journals/np/2016/5108489/abs/
4.TJ Song.Altered Behaviors and Impaired Synaptic Function in a Novel Rat Model With a Complete Shank3 Deletion.
文献链接：https://www.frontiersin.org/articles/10.3389/fncel.2019.00111/full
5.CI Choi,etal.The progeroid gene BubR1 regulates axon myelination and motor function.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191862/pdf/aging-08-2667.pdf
6.李娇.三七总皂苷、栀子苷及其配伍对APP/PS1小鼠树突棘损伤的保护作用及机制.
文献链接：
https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CMFD&dbname=CMFD201402&filename=1014242066.nh&v=MDE2NzZXci9NVkYyNkdyRzhITkhLcVpFYlBJUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWWVScUZ5L2c=
7.柯鑫,双酚A早期暴露对雄性大鼠成年后海马区少突胶质细胞和恐惧学习的影响.
文献链接：
https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CMFD&dbname=CMFD201601&filename=1015627684.nh&v=MDk4NDIyNkc3VzZHZGZFcTVFYlBJUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWWVScUZ5L2dXNy9BVkY=
8.W Duan.Deletion of Tbk1 disrupts autophagy and reproduces behavioral and locomotor symptoms of FTD-ALS in mice.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519994/
9.Qiang Liu.Dcf1 Triggers Dendritic Spine Formation and Facilitates Memory Acquisition.
文献链接：https://link.springer.com/article/10.1007/s12035-016-0349-6
10.YuanWang.Maternal iodine supplementation improves motor coordination in offspring by modulating the mGluR1 signaling pathway in mild iodine deficiency-induced hypothyroxinemia rats.
文献链接：https://www.sciencedirect.com/science/article/pii/S0955286318300688
11.Hao Huang.Protein expression of phospho-lim kinase-1 in patients and an experimental rat model with intractable temporal lobe epilepsy.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358492/
12.K Zhang.ALK5 signaling pathway mediates neurogenesis and functional recovery after cerebral ischemia/reperfusion in rats via Gadd45b.
文献链接：https://www.nature.com/articles/s41419-019-1596-z
13.X Huang.The development of the serotonergic and dopaminergic systems during chicken mid-late embryogenesis.
文献链接：https://www.sciencedirect.com/science/article/pii/S0303720719301741
14 Hui Min.Marginal Iodine Deficiency Affects Dendritic Spine Development by Disturbing the Function of Rac1 Signaling Pathway on Cytoskeleton.
文献链接：https://link.springer.com/article/10.1007/s12035-015-9657-5
15.Y Yu.Maternal marginal iodine deficiency delays cerebellar Bergmann glial cell development in rat offspring: Involvement of Notch signaling pathway.
文献链接：https://www.sciencedirect.com/science/article/pii/S0161813X18303292
关键词
高尔基染色试剂盒，高尔基，Golgi Staining,Hitobiotec Corp.；Golgi-Cox impregnation 浸染法；neuron 神经元（神经细胞）；glia 神经胶质（胶质细胞）
产品描述
Golgi-Cox 染色或Golgi-Cox 浸染法（Golgi-Cox impregnation）公认为是用来研究神经元和神经胶质细胞形态的最有效和美观的通用方法之一。近些年高尔基体染色Golgi-Cox 染色法依然是用来检查树突分枝模式和树突棘的优选方法，因其能对大脑某特定区域随机挑取的少数片段内的树突树（dendritic arbour）进行分离和鉴定。
高尔基染色技术不仅只用于解剖研究，而且广泛用于行为-形态关系研究。一些研究中Golgi-Cox法曾用来鉴定和研究心脏自主神经支配，结果显示Golgi-Cox法是一种非常有效的技术来鉴定和研究外周血组织自主神经支配的形态学特征。然而，Golgi-Cox染色法很费时，需要使用振动切片机，细胞染色率通常比较低，结果常常不可靠。
Hitobiotec Corp.公司生产的高尔基体染色试剂盒（Hito Golgi-Cox OptimStain™ Kit）针对以上问题提供了一种简单的解决方案。基于Glaser and Van der Loos描述的方法，本试剂盒对Golgi-Cox 技术做出了显著改善。此试剂盒步骤简单且操作时间明显降低。科研人员可使用-19ºC保存的冷冻切片机（cryostat）。不仅提供稳定和改善的染色质量，而且只要适当操作几乎无过度染色。标准试剂盒可用于50个小鼠脑切片染色。
如果没有冷冻切片机（cryostat），组织可用薄片切片机（microtome）制备成石蜡切片。
Hitobiotec Corp.公司生产的高尔基体染色试剂盒（Hito Golgi-Cox OptimStain™ Kit）经测试广泛用于不同动物的脑、脊髓和心脏组织，以及高灵敏度显示神经元和神经胶质细胞的形态学细节。
试剂盒组分
| 组分 | Standard Kit | Small Kit |
| Solution-1A (Stock Solution) 溶液-1A | 100 ml | 50 ml |
| Solution-1B (Prepare Solution) 溶液-1B | 150 ml | 75 ml |
| Solution-2 溶液2 | 250 ml | 125 ml |
| Solution-3 溶液3 | 500 ml | 250 ml |
| Solution-4 溶液4 | 250 ml | 125 ml |
| Solution-5 溶液5 | 250 ml | 125 ml |
| Dropper Bottle (30 ml Solution-3) 滴瓶 | 1 | 1 |
| Staining Jar (12 ml) 染缸 | 2 | 1 |
| Fine Tip Natural Hair Brush | 2 | 1 |
| Glass Specimen Transfer Tool | 2 | 1 |
| User Manual and MSDS | 1 | 1 |
染色结果示例：
| 品牌 | 名称 | 货号 | 备注 |
| Hitobiotec | Hito Golgi-Cox Optimstain kit | HTKNS1125 | 完全版 |
| Hitobiotec | Hito Golgi-Cox OptimStain PreKit | HTKNS1125NH | 安全运输版 |
注意：
Hito Golgi-Cox OptimStain PreKit，这个是安全运输版试剂，客户参照说明书中溶液1中需要添加的化学组分，自行购买相关试剂，盖紧瓶子剧烈摇晃十几分钟溶解后就和第一种完全版试剂盒一样了，这个试剂盒是无害的，可以用普通包装邮寄。
引用文献：
引用文献：
1.BH Kan,etal.Acupuncture improves dendritic structure and spatial learning and memory ability of Alzheimer's disease mice.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108219/
2.ZhiqiangWang,etal.Chronic cerebral hypoperfusion induces long-lasting cognitive deficits accompanied by long-term hippocampal silent
synapses increase in rats.
文献链接：https://doi.org/10.1016/j.bbr.2015.12.047
3.J Yuan, H Huang,etal.MicroRNA-132 interact with p250GAP/Cdc42 pathway in the hippocampal neuronal culture model of acquired epilepsy and associated with epileptogenesis process.
文献链接：https://www.hindawi.com/journals/np/2016/5108489/abs/
4.TJ Song.Altered Behaviors and Impaired Synaptic Function in a Novel Rat Model With a Complete Shank3 Deletion.
文献链接：https://www.frontiersin.org/articles/10.3389/fncel.2019.00111/full
5.CI Choi,etal.The progeroid gene BubR1 regulates axon myelination and motor function.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191862/pdf/aging-08-2667.pdf
6.李娇.三七总皂苷、栀子苷及其配伍对APP/PS1小鼠树突棘损伤的保护作用及机制.
文献链接：
https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CMFD&dbname=CMFD201402&filename=1014242066.nh&v=MDE2NzZXci9NVkYyNkdyRzhITkhLcVpFYlBJUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWWVScUZ5L2c=
7.柯鑫,双酚A早期暴露对雄性大鼠成年后海马区少突胶质细胞和恐惧学习的影响.
文献链接：
https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CMFD&dbname=CMFD201601&filename=1015627684.nh&v=MDk4NDIyNkc3VzZHZGZFcTVFYlBJUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWWVScUZ5L2dXNy9BVkY=
8.W Duan.Deletion of Tbk1 disrupts autophagy and reproduces behavioral and locomotor symptoms of FTD-ALS in mice.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519994/
9.Qiang Liu.Dcf1 Triggers Dendritic Spine Formation and Facilitates Memory Acquisition.
文献链接：https://link.springer.com/article/10.1007/s12035-016-0349-6
10.YuanWang.Maternal iodine supplementation improves motor coordination in offspring by modulating the mGluR1 signaling pathway in mild iodine deficiency-induced hypothyroxinemia rats.
文献链接：https://www.sciencedirect.com/science/article/pii/S0955286318300688
11.Hao Huang.Protein expression of phospho-lim kinase-1 in patients and an experimental rat model with intractable temporal lobe epilepsy.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358492/
12.K Zhang.ALK5 signaling pathway mediates neurogenesis and functional recovery after cerebral ischemia/reperfusion in rats via Gadd45b.
文献链接：https://www.nature.com/articles/s41419-019-1596-z
13.X Huang.The development of the serotonergic and dopaminergic systems during chicken mid-late embryogenesis.
文献链接：https://www.sciencedirect.com/science/article/pii/S0303720719301741
14 Hui Min.Marginal Iodine Deficiency Affects Dendritic Spine Development by Disturbing the Function of Rac1 Signaling Pathway on Cytoskeleton.
文献链接：https://link.springer.com/article/10.1007/s12035-015-9657-5
15.Y Yu.Maternal marginal iodine deficiency delays cerebellar Bergmann glial cell development in rat offspring: Involvement of Notch signaling pathway.
文献链接：https://www.sciencedirect.com/science/article/pii/S0161813X18303292
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
用户评价
暂无用户评价
文献和实验该产品被引用文献
1.BH Kan,etal.Acupuncture improves dendritic structure and spatial learning and memory ability of Alzheimer's disease mice.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108219/
2.ZhiqiangWang,etal.Chronic cerebral hypoperfusion induces long-lasting cognitive deficits accompanied by long-term hippocampal silent
synapses increase in rats.
文献链接：https://doi.org/10.1016/j.bbr.2015.12.047
3.J Yuan, H Huang,etal.MicroRNA-132 interact with p250GAP/Cdc42 pathway in the hippocampal neuronal culture model of acquired epilepsy and associated with epileptogenesis process.
文献链接：https://www.hindawi.com/journals/np/2016/5108489/abs/
4.TJ Song.Altered Behaviors and Impaired Synaptic Function in a Novel Rat Model With a Complete Shank3 Deletion.
文献链接：https://www.frontiersin.org/articles/10.3389/fncel.2019.00111/full
5.CI Choi,etal.The progeroid gene BubR1 regulates axon myelination and motor function.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191862/pdf/aging-08-2667.pdf
6.李娇.三七总皂苷、栀子苷及其配伍对APP/PS1小鼠树突棘损伤的保护作用及机制.
文献链接：
https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CMFD&dbname=CMFD201402&filename=1014242066.nh&v=MDE2NzZXci9NVkYyNkdyRzhITkhLcVpFYlBJUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWWVScUZ5L2c=
7.柯鑫,双酚A早期暴露对雄性大鼠成年后海马区少突胶质细胞和恐惧学习的影响.
文献链接：
https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CMFD&dbname=CMFD201601&filename=1015627684.nh&v=MDk4NDIyNkc3VzZHZGZFcTVFYlBJUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWWVScUZ5L2dXNy9BVkY=
8.W Duan.Deletion of Tbk1 disrupts autophagy and reproduces behavioral and locomotor symptoms of FTD-ALS in mice.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519994/
9.Qiang Liu.Dcf1 Triggers Dendritic Spine Formation and Facilitates Memory Acquisition.
文献链接：https://link.springer.com/article/10.1007/s12035-016-0349-6
10.YuanWang.Maternal iodine supplementation improves motor coordination in offspring by modulating the mGluR1 signaling pathway in mild iodine deficiency-induced hypothyroxinemia rats.
文献链接：https://www.sciencedirect.com/science/article/pii/S0955286318300688
11.Hao Huang.Protein expression of phospho-lim kinase-1 in patients and an experimental rat model with intractable temporal lobe epilepsy.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358492/
12.K Zhang.ALK5 signaling pathway mediates neurogenesis and functional recovery after cerebral ischemia/reperfusion in rats via Gadd45b.
文献链接：https://www.nature.com/articles/s41419-019-1596-z
13.X Huang.The development of the serotonergic and dopaminergic systems during chicken mid-late embryogenesis.
文献链接：https://www.sciencedirect.com/science/article/pii/S0303720719301741
14 Hui Min.Marginal Iodine Deficiency Affects Dendritic Spine Development by Disturbing the Function of Rac1 Signaling Pathway on Cytoskeleton.
文献链接：https://link.springer.com/article/10.1007/s12035-015-9657-5
15.Y Yu.Maternal marginal iodine deficiency delays cerebellar Bergmann glial cell development in rat offspring: Involvement of Notch signaling pathway.
文献链接：https://www.sciencedirect.com/science/article/pii/S0161813X18303292
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108219/
2.ZhiqiangWang,etal.Chronic cerebral hypoperfusion induces long-lasting cognitive deficits accompanied by long-term hippocampal silent
synapses increase in rats.
文献链接：https://doi.org/10.1016/j.bbr.2015.12.047
3.J Yuan, H Huang,etal.MicroRNA-132 interact with p250GAP/Cdc42 pathway in the hippocampal neuronal culture model of acquired epilepsy and associated with epileptogenesis process.
文献链接：https://www.hindawi.com/journals/np/2016/5108489/abs/
4.TJ Song.Altered Behaviors and Impaired Synaptic Function in a Novel Rat Model With a Complete Shank3 Deletion.
文献链接：https://www.frontiersin.org/articles/10.3389/fncel.2019.00111/full
5.CI Choi,etal.The progeroid gene BubR1 regulates axon myelination and motor function.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191862/pdf/aging-08-2667.pdf
6.李娇.三七总皂苷、栀子苷及其配伍对APP/PS1小鼠树突棘损伤的保护作用及机制.
文献链接：
https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CMFD&dbname=CMFD201402&filename=1014242066.nh&v=MDE2NzZXci9NVkYyNkdyRzhITkhLcVpFYlBJUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWWVScUZ5L2c=
7.柯鑫,双酚A早期暴露对雄性大鼠成年后海马区少突胶质细胞和恐惧学习的影响.
文献链接：
https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CMFD&dbname=CMFD201601&filename=1015627684.nh&v=MDk4NDIyNkc3VzZHZGZFcTVFYlBJUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWWVScUZ5L2dXNy9BVkY=
8.W Duan.Deletion of Tbk1 disrupts autophagy and reproduces behavioral and locomotor symptoms of FTD-ALS in mice.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519994/
9.Qiang Liu.Dcf1 Triggers Dendritic Spine Formation and Facilitates Memory Acquisition.
文献链接：https://link.springer.com/article/10.1007/s12035-016-0349-6
10.YuanWang.Maternal iodine supplementation improves motor coordination in offspring by modulating the mGluR1 signaling pathway in mild iodine deficiency-induced hypothyroxinemia rats.
文献链接：https://www.sciencedirect.com/science/article/pii/S0955286318300688
11.Hao Huang.Protein expression of phospho-lim kinase-1 in patients and an experimental rat model with intractable temporal lobe epilepsy.
文献链接：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358492/
12.K Zhang.ALK5 signaling pathway mediates neurogenesis and functional recovery after cerebral ischemia/reperfusion in rats via Gadd45b.
文献链接：https://www.nature.com/articles/s41419-019-1596-z
13.X Huang.The development of the serotonergic and dopaminergic systems during chicken mid-late embryogenesis.
文献链接：https://www.sciencedirect.com/science/article/pii/S0303720719301741
14 Hui Min.Marginal Iodine Deficiency Affects Dendritic Spine Development by Disturbing the Function of Rac1 Signaling Pathway on Cytoskeleton.
文献链接：https://link.springer.com/article/10.1007/s12035-015-9657-5
15.Y Yu.Maternal marginal iodine deficiency delays cerebellar Bergmann glial cell development in rat offspring: Involvement of Notch signaling pathway.
文献链接：https://www.sciencedirect.com/science/article/pii/S0161813X18303292
相关实验
。正常情况下，它存在于线粒体内膜和外膜之间的腔中，凋亡信号刺激使其从线粒体释放至细胞液，结合Apaf-1 （apoptotic protease activating factor-1）后启动caspase级联反应：细胞色素C/Apaf-1复合物激活caspase-9，后者再激活caspase-3和其它下游caspase。细胞色素C氧化酶亚单位Ⅳ（cytochrome c oxidase subunit Ⅳ：COX4）是定位在线粒体内膜上的膜蛋白，凋亡发生时，它保留在线粒体内，因而它是线粒体富集
研选
高尔基染色试剂盒 Hitobiotec Golgi-Cox OptimStain™ Prekit
￥5900
