- ¥9120
- ACTIFLUOR
- 美国
- 812
- 2025年12月26日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
American Diagnostica Inc
- 检测方法：
FRET
- 应用：
枸橼酸钠抗凝血浆
- 标记物：
ALEXA488-VWF86 FRET Substrate
- 样本：
20 μl
- 规格：
48 tests
血管性血友病因子裂解酶ADAMTS-13活性试剂盒
INTENDED USE
The ACTIFLUOR™ ADAMTS13 Activity assay is a fluorescence resonance energy transfer(FRET) assay for the measurement of ADAMTS13 in human plasma. The assay is for in vitro diagnostic use.
PRINCIPLE OF THE METHOD
ACTIFLUOR ADAMTS13 is a FRET assay that measures the amount of ADAMTS13 activity in human plasma. A citrated plasma sample is assayed for ADAMTS13 protease activity using recombinant VWF86-ALEXA FRET substrate. Proteolytic cleavage of the VWF86-ALEXA FRET substrate between the tyr/met residues by ADAMTS13 uncouples the ALEXA fluorochromes resulting in an increase in fluorescence. The increase in fluorescence over time (Vmax) due to cleavage of the substrate by ADAMTS13 is monitored at 37°C using a spectrofluorometer (Ex=485 nm; Em=535 nm). A standard curve is constructed using normal plasma with a known concentration of ADAMTS13. The ADAMTS13 activity in the plasma is determined by interpolation of the Vmax values from the standard curve.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验Alanine Transaminase Activity Assay
for the fluorometric assay. 3) Sample Preparations: Tissues (50 mg) or cells (1×106) can be homogenized in ~ 200 µl ice-cold Alanine Transaminase Assay Buffer, then centrifuged (13,000 g, 10 min.) to remove insoluble material. Serum samples
CANDOR BIOSCIENCE: 关于ELISA板稳定性的技术探讨与比较
物稳定剂，例如CANDOR的Antibody Stabilizer、HRP-Protector™和AP-Protector®，可以大大提高对照品和检测抗体-结合物的稳定性。本文以ELISAs为例，重点讨论固相稳定、表面包被捕获分子的活性维持的机理和解决方案。此外，本文概述的原理和解决方案也可以应用于其他包被表面，如聚苯乙烯或玻璃表面的实验应用，如侧向层析、亲和柱、基于微球的分析、免疫PCR或蛋白质分析等。 包被的捕获分子如抗体，或在抗原下降分析中分离的病毒蛋白，是可靠免疫分析的核心组成
Correlation of Caspase Activity and In Vitro Chemo-Response in Epithelial Ovarian Cancer Cell Lines
The immediate assessment of response to therapy is most beneficial to ovarian cancer patients. This study shows the correlation of drug-induced caspase activation determined by western blot analysis and by Caspase-Glo™ assay. Our findings
技术资料暂无技术资料 索取技术资料
