AssayPro—Human uPA Chromogenic

Introduction
Urokinase-type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodeling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation (1, 2). High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer (3, 4, 5).

Principle of Assay
The AngioSense Human uPA Chromogenic Activity Assay Kit is developed to determine human uPA activity in plasma and cell culture supernatants. The amidolytic activity of uPA is quantitated using a highly specific uPA substrate releasing a (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the uPA enzymatic activity.