ZYMUPHEN Microparticles' Proco

Product Description
Functional assay for the measurement of microparticles’ procoagulant activity in human plasma (through thrombin generation) using automated or manual method.

INTENDED USE:
The ZYMUPHEN MP-Activity kit is a functional assay proposed for the measurement of microparticles’ procoagulant activity in human plasma, in vitro, using automated or manual method.

APPLICATIONS:
Assay of microparticles in some clinical contexts such as cardiovascular diseases, malignancy,
infectious or inflammatory pathology.

ASSAY PRINCIPLE:
The diluted assayed plasma sample, supplemented with calcium, Factor Xa and thrombin inhibitors, is introduced into one of the microplate wells coated with Streptavidine and biotinylated Annexin V, then incubated. Following a washing step, the Factor Xa-Va mixture containing calcium, then the purified prothrombin, are introduced. When present in the tested sample, microparticles bind to Annexin V and expose their phospholipids surface, thus allowing to FXa-FVa, in presence of calcium, to activate prothrombin into thrombin. The phospholipids concentration is then the limiting factor. There is a direct relationship between the phospholipids concentration and the amount of thrombin generation, which is measured via its specific activity on the thrombin substrate. The reaction is stopped with 2% Citric Acid and Absorbance is measured at 405 nm (A405).

Assay Characteristics	Kit Components
Total Assay Time: < 1h25 Detection Threshold: ≤ 0.05 nM Dynamic Range: 0 to 50 nM Intra-Assay CV: 3-8% Inter-Assay CV: 5-10%	12x8 test kit (coated with Annexin V) 2x Sample Diluent R1: 2x Bovine FXa-FVa mixture R2: 2x purified human Prothrombin R3: 2x Thrombin specific chromogenic substrate 2x Calibrator 1x Control (CI & CII) 1x Wash Solution 1x 2% Citric Acid