Mouse ADAM metallopeptidase wi

Mouse ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13) ELISA kit

标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected
5000 2.401 2.476 2.439 2.337
2500 1.884 1.875 1.880 1.778
1250 1.413 1.424 1.419 1.317
625 0.777 0.798 0.788 0.686
312 0.466 0.476 0.471 0.369
156 0.265 0.258 0.262 0.160
78 0.176 0.184 0.180 0.078
0 0.101 0.103 0.102

Mouse ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13) ELISA kit

精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Mouse ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13) ELISA kit

线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAMTS13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1000 Average % 95
Range % 89-98
1:2000 Average % 92
Range % 88-95
1:4000 Average % 92
Range % 85-95
1:8000 Average % 98
Range % 92-101

Mouse ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13) ELISA kit

回收率:
The recovery of mouse ADAMTS13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 86-94
EDTA plasma (n=4) 103 98-107