pSPT18

pSPT18

质粒名称: pSPT18
其他名称:
质粒大小（bp）: 3104
抗性:氨苄青霉素Amp
质粒类型: 大肠杆菌载体；克隆质粒
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
质粒图谱:

 

质粒序列:

LOCUS       Exported                3104 bp ds-DNA     circular SYN 12-十二月-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSPT18
SOURCE      synthetic DNA construct
ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3104)
AUTHORS   .
TITLE     Direct Submission
JOURNAL   Exported 2016-12-12 from SnapGene Viewer 2.8.1
http://www.miaolingbio.com
FEATURES             Location/Qualifiers
source          1..3104
/organism=”synthetic DNA construct”
/mol_type=”other DNA”
misc_feature    7..63
/note=”MCS”
/note=”pUC18/19 multiple cloning site”
promoter        complement(71..89)
/note=”T7 promoter”
/note=”promoter for bacteriophage T7 RNA polymerase”
misc_feature    248..390
/note=”bom”
/note=”basis of mobility region from pBR322″
rep_origin      complement(576..1164)
/direction=LEFT
/note=”ori”
/note=”high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication”
CDS             complement(1335..2195)
/codon_start=1
/gene=”bla”
/product=”beta-lactamase”
/note=”AmpR”
/note=”confers resistance to ampicillin, carbenicillin, and
related antibiotics”
/translation=”MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW”
promoter        complement(2196..2300)
/gene=”bla”
/note=”AmpR promoter”
promoter        3088..2
/note=”SP6 promoter”
/note=”promoter for bacteriophage SP6 RNA polymerase”
ORIGIN
1 gaatacaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
61 ttccggtctc cctatagtga gtcgtattaa tttcgataag ccagctgggc ctcgcgcgtt
121 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
181 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
241 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatata ctggcttaac
301 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
361 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
1021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
1081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
1141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
1201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
1261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
1321 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
1381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
1441 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
1501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
1561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
1621 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
1681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
1741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
1801 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
1861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
1921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
1981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
2041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
2101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
2161 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
2221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
2281 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
2341 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
2401 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
2461 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
2521 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
2581 atcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
2641 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
2701 cggccacggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
2761 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
2821 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct
2881 gattggttcg ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg
2941 tccaaccaaa ccgactctga cggcagttta cgagagagat gatagggtct gcttcagtaa
3001 gccagatgct acacaattag gcttgtacat attgtcgtta gaacgcggct acaattaata
3061 cataacctta tgtatcatac acatacgatt taggtgacac tata
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