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- PVT10566
- 2025年09月20日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pSPT18
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pSPT18
Catalog No. PVT10566
Packing 2ug
pSPT18 Information
Function E.coli Cloning plasmid
Promoter: T7 promoter
Replicon: PBR322 ori
Plasmid classification: Escherichia coli vector; cloned plasmid
Plasmid size: 3104bp
Prokaryotic resistance: ampicillin Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: BL21 (DE3)
Culture conditions: 37 centigrade, aerobic, LB
pSPT18 Description
PSPT18 is a high copy plasmid, plasmid length 3104bp, contains Amp penicillin resistance gene, replicon is pBR322 ori.
pSPT18 Sequence
LOCUS Exported 3104 bp ds-DNA circular SYN 12-DEC-2016
DEFINITION synthetic circular DNA
KEYWORDS pSPT18
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3104)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3104
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 7..63
/note="MCS"
/note="pUC18/19 multiple cloning site"
promoter complement(71..89)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 248..390
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(576..1164)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1335..2195)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2196..2300)
/gene="bla"
/note="AmpR promoter"
promoter 3088..2
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
1 gaatacaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
61 ttccggtctc cctatagtga gtcgtattaa tttcgataag ccagctgggc ctcgcgcgtt
121 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
181 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
241 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatata ctggcttaac
301 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
361 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
1021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
1081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
1141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
1201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
1261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
1321 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
1381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
1441 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
1501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
1561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
1621 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
1681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
1741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
1801 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
1861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
1921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
1981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
2041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
2101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
2161 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
2221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
2281 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
2341 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
2401 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
2461 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
2521 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
2581 atcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
2641 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
2701 cggccacggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
2761 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
2821 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct
2881 gattggttcg ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg
2941 tccaaccaaa ccgactctga cggcagttta cgagagagat gatagggtct gcttcagtaa
3001 gccagatgct acacaattag gcttgtacat attgtcgtta gaacgcggct acaattaata
3061 cataacctta tgtatcatac acatacgatt taggtgacac tata
//
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文献和实验This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
质粒 plasmid 在细胞内整个世代稳定地传递给后代，但并不在染色体上而进行自主增殖的一类遗传因子的总称。该类因子的存在与否，对细胞的生存无关紧要。这个名词主要指细菌细胞内的因子，它们包括可在细胞间发生传递的F因子、 R因子、大肠杆菌素因子等。此外，还有各种本身不能传递的因子。在具有质粒细胞的后代中，有时会出现低频率的失去质粒的细菌。在质粒中，对那些能变成插入奇主染色体状态的特殊质粒，特称为附加体（episome）。
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