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- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pSicoR-Ef1a-mCh-Puro(pSicoREf1amChPuro)
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
质粒名称: pSicoR-Ef1a-mCh-Puro
其他名称:
质粒大小（bp）: 8141
抗性: Ampicillin
质粒类型: Mammalian Expression, Lentiviral, RNAi, Cre/Lox
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
质粒图谱:
质粒序列:
>pSicoR-Ef1a-mCh-Puro sequence 8141 bps
gcttaagcggtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctga
tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaa
atttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttg
cgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatc
aattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccg
cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaa
tagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagt
gtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccag
tacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtga
tgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccc
cattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactcc
gccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgt
actgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctta
agcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaacta
gagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaag
cgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcga
ggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgag
agcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaaga
aaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcct
gttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaa
gaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagaca
ccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgg
ccgcgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataa
agtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaa
agagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgt
caatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgag
ggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatc
ctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcattt
gcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgac
ctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaa
aaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggttta
acataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaat
agtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccac
ctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagaca
gatccattcgattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatcc
acaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaac
agacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggac
agcagagatccagtttggttagtaccgggcccgctctagagatccgacgcgccatctctaggcccgcgcc
ggccccctcgcacagacttgtgggagaagctcggctactcccctgccccggttaatttgcatataatatt
tcctagtaactatagaggcttaatgtgcgataaaagacagataatctgttctttttaatactagctacat
tttacatgataggcttggatttctataacttcgtatagcatacattatacgaagttatacatgtcacaaa
aggaaactcaccctaactgtaaagtaattgtgtgttttgagactataaatatcccttggagaaaagcctt
gttaacgcgcggtgaccctcgagtactaggatccattaggcggccgcggatctgcgatcgctccggtgcc
cgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccg
gtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccga
gggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgcc
agaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgcc
atccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtcta
ggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagc
cggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgcc
gttacagatccaagctgtgaccggcgcctacgctagcgctaccggtcgccaccatggtgagcaagggcga
ggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggc
cacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaagg
tgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggc
ctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggag
cgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagt
tcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccat
gggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagagg
ctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgc
agctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgt
ggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagccccgggag
ggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctcgaaccgagtacaagccca
cggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgacta
ccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttc
ctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctgga
ccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcgg
ttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtgg
ttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccg
gagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctcccctt
ctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatg
acccgcaagcccggtgcctaagaattcgtcgagggacctaataacttcgtatagcatacattatacgaag
ttatacatgtttaagggttccggttccactaggtacaattcgatatcaagcttatcgataatcaacctct
ggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatac
gctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaat
cctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtt
tgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttc
cccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgt
tgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgc
cacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcc
cgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccc
tttgggccgcctccccgcatcgataccgtcgacctcgatcgagacctagaaaaacatggagcaatcacaa
gtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttt
tccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccacttttta
aaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatatccttgatctgtggatct
accacacacaaggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgac
ctttggatggtgctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaac
acccgcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtattagagtggaggt
ttgacagccgcctagcatttcatcacatggcccgagagctgcatccggactgtactgggtctctctggtt
agaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttg
ccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagaccct
tttagtcagtgtggaaaatctctagcagcatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaa
ggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagt
cagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgct
ctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttc
tcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaa
ccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacg
acttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacaga
gttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaag
ccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt
tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctac
ggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatc
ttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggt
ctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagt
tgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatg
ataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagc
gcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaag
tagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcg
tttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgca
aaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcat
ggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgag
tactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacggg
ataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaact
ctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagca
tcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataa
gggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggtta
ttgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt
ccccgaaaagtgccacctgac
>U6 (cagtgcaggggaaagaatagtagac)
GCNGNATCAACTAaAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTACAGGGACA
GCAGAGATCCAGTTTGGTTAGTACCGGGCCCGCTCTAGAGATCCGACGCGCCATCTCTAGGCCCGCGCCG
GCCCCCTCGCACAGACTTGTGGGAGAAGCTCGGCTACTCCCCTGCCCCGGTTAATTTGCATATAATATTT
CCTAGTAACTATAGAGGCTTAATGTGCGATAAAAGACAGATAATCTGTTCTTTTTAATACTAGCTACATT
TTACATGATAGGCTTGGATTTCTATAACTTCGTATAGCATACATTATACGAAGTTATACATGTCACAAAA
GGAAACTCACCCTAACTGTAAAGTAATTGTGTGTTTTGAGACTATAAATATCCCTTGGAGAAAAGCCTTG
TTAACGCGCGGTGACCCTCGAGTACTAGGATCCATTAGGCGGCCGCGGATCTGCGATCGCTCCGGTGCCC
GTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGG
TGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAG
GGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCA
GAACACAGCTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGAGGCCGCCA
TCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCCTCCTGAACTGCGTCCGCCGTCTAG
GTAAGTTTAAAGCTCAGGTCGAGACCGGGCCTTTGTCCGGCGCTCCCTtGGAGCCTACCTAGACTCAGCC
GGCTCTCcACGCTTTGCCTG
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文献和实验1.常用载体分类：2,常用载体元件介绍：(1) 启动子：启动子是位于结构基因5'端上游的DNA序列，能活化RNA聚合酶，使之与模板DNA准确的结合并具有转录起始的特异性。启动子主要分为广谱启动子和特异性启动子。广谱启动子为在各种组织、细胞上广泛表达的启动子；特异性启动子为在特异性组织或者细胞类型上表达的启动子。常用广谱启动子有CAG，CMV，EF1a，PGK等，还包含启动RNA的III型启动子U6和H1启动子；常用特异性启动子如成熟神经元特异性启动子hSyn，投射神经元特异性启动
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