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- 默克
- USA
- 444810
- 2025年09月18日
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- 英文名：
ProteoExtract® Native Membrane Protein Extraction Kit
- 供应商：
研卉生物
- 规格：
1 kit
英文名称：ProteoExtract® Native Membrane Protein Extraction Kit
| Description | |
|---|---|
| Overview | A convenient kit for the isolation of native integral membrane and membrane-associated proteins, without the need for ultracentrifugation. Extraction is based on association of proteins with cellular membranes rather than on their hydrophobicity. Resulting samples are suitable for use in functional assays, 2-D gel electrophoresis, and other applications. |
| Note: 1 kit is sufficient for up to 20 sample extractions. | |
| Catalogue Number | 444810 |
| Brand Family | Calbiochem® |
| Synonyms | M-PEK Kit |
| Materials Required but Not Delivered | • Platform mixer e.g., IKA Vibramax (when extracting adherent tissue culture cells) • Cell culture equipment, media etc. for cell growth (e. g., RPMI, DMEM). • Micropipettes and tips, 10 µl, 200 µl and 1 ml size (e.g., Eppendorf, Gilson or equivalent) • Cooled centrifuge and rotor for 50 or 15 ml tube size (Eppendorf, Heraeus, Nalgene, etc.) • Cooled micro centrifuge and rotor up to 18,000 x g for 2 ml tube size (e.g. Eppendorf) • Thermo mixer or rolling facility (e.g., Eppendorf) • Homogenizer (e.g. potter or Dounce) or mortar. |
| Product Information | |
|---|---|
| Form | 20 Extractions |
| Kit contains | Wash Buffer, Extraction Buffer 1, Extraction Buffer 2, Protease Inhibitor Cocktail, and a user protocol. |
| Biological Information | |
|---|---|
| Sample Type | Mammalian tissues and cultured cells |
| Product Usage Statements | |
|---|---|
| Intended use | The ProteoExtract™ Native Membrane Protein Extraction Kit is designed for the isolation of native membrane proteins from a broad range of mammalian samples, including: • Adherent tissue culture cells • Suspension grown tissue culture cells • Frozen cell pellets • Tissues |
| Storage and Shipping Information | |
|---|---|
| Ship Code | Shipped at Ambient Temperature or with Blue Ice |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | +2°C to +8°C |
| Storage Conditions | • Wash Buffer Store at 4°C. • Extraction Buffers I & II The Extraction Buffers I and II can be stored at 4°C for up to 6 month. For prolonged storage, freeze the buffers in convenient aliquots at -20°C. Before extraction, buffers must be thawed at room temperature (RT). After thawing, mix components by gently shaking or vortexing. Avoid repeated freezing and thawing! • Protease Inhibitor Cocktail The Protease Inhibitor Cocktail is supplied in DMSO and can be stored at 4°C up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C. During the sample preparation procedure it must be kept at RT to prevent freezing of DMSO. |
| Do not freeze | Ok to freeze |
特点：
3-5倍富集天然膜蛋白
得到天然、有活性的蛋白
两步操作，仅需1至1.5小时
可平行处理多个样本
无需超高速离心
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文献和实验跨膜区的膜蛋白）的效果往往很差。下面推荐的是两个公司的相关产品，也算是具有代表性的两种方法： 1． 默克的跨膜蛋白抽提试剂盒ProteoExtract 跨膜蛋白抽提试剂盒（简称TM-PEK，货号71772-3）是一种基于化学而非去污剂方法的高产膜蛋白制备试剂盒。其简便的两步法操作可以高效地富集膜蛋白和膜关联蛋白。试剂盒包括两种试剂，TM-PEK试剂A和TM-PEK试剂B，分别用于制备抽提缓冲液2A和抽提缓冲液2B。使用者通过实验摸索，可以根据特定目的蛋白的特点从中灵活选择最适缓冲
人载脂蛋白M (APO M)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 APO M 单抗包被于酶标板上，标准品和样品中的 APO M与单抗结合，加入生物素化的抗人 APO M ，形成免疫复合物连接在板上，辣根
992)样本：人 HeLa 细胞 (Fig.6)A. 5% 脱脂奶粉封闭，一抗浓度：1.0 mg/mL，溶于 5% 脱脂奶粉和 0.15 M NaClB. 5% 脱脂奶粉封闭，一抗浓度：1.0 mg/mL，溶于 5% 脱脂奶粉和 0.5 M NaClFig.6我们为您推荐实验设计数据参考：裂解缓冲液蛋白定位裂解液推荐试剂盒全细胞NP-40 or RIPA全蛋白抽提试剂盒细胞质（可溶蛋白）Tris-HCl胞浆蛋白和核蛋白抽提试剂盒线粒体蛋白提取试剂盒细胞质（细胞骨架 等不溶蛋白）Tris
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