pKM1

pKM1

质粒名称: pKM1
其他名称: 
质粒大小（bp）: 4378
抗性: Ampicillin 
质粒类型: Unspecified
是否病毒: 
启动子: 
稳转或瞬转: 
组成型或诱导性: 
表达水平: 
蛋白标签: 
测序引物: 
GenBank号: L08921 
其他属性: This is a promoter probe vector. The promoterless galK gene is used as selectable marker. Translationsal stop codons in all reading frames are present on a 11-bp oligonucleotide before the galKgene. Also, a polylinker was cloned before this gene. In addition, the lambda tr1 transcription terminator was inserted BEFORE the galK gene to allow measurement of very strong promoters due to read-through transcription. Promoter-cloning vector for isolating and characterizing very strong promoters. [1] This vector was constructed by inserting a rho-dependent terminator (lambdaTRI) into the SmaI site of pKO1 (ATCC 37126). This reduces the number of transcripts reaching the galK coding sequences to 30% or less & is thus useful for strong promoters. [2] Medium is 1227 LB plus ampicillin. Hosts: E.coli N100, E.coli. Related vectors: EcoGalK, pBR322, pKO-1, lambda. 
质粒图谱:

 

 

质粒序列:

1	ttctggcgaa	tcctctgacc	agccagaaaa	cgacctttct	gtggtgaaac	50
51	cggatgctgc	aattcagagc	gccagcaagt	gggggacagc	agaagacctg	100
101	accgccgcag	agtggatgtt	tgacatggtg	aagactatcg	caccatcagc	150
151	cagaaaaccg	aattttgctg	ggtgggctaa	cgatatccgc	ctgatgcgtg	200
201	aacgtgacgg	acgtaaccac	cgcgacatgt	gtgtgctgtt	ccgctgggca	250
251	tgccaggaca	acttctggtc	cggtaacgtg	ctgagcccgg	ccaagcttac	300
301	tccccatccc	cccgaaagat	ttttttaact	ataaacgctg	atggaagcgt	350
351	ttatgcggaa	gaggtaaagc	ccttcccgag	taacaaaaaa	acaacagcat	400
401	aaataacccc	gctcttacac	attccagccc	tgaaaaaggg	catcaaatta	450
451	aaccacacct	atggtgtatg	catttatttg	catacattca	atcaattgtt	500
501	atctaaggaa	atacttacat	atggttcgtg	caaacaaacg	caacgaggct	550
551	ctacgaatcg	agagtgcgtt	gcttaacaaa	atcgcaatgc	ttggaactga	600
601	gaagacagcg	gaagctgtgg	gcgttgataa	gtcgcagatc	agcaggtgga	650
651	agagggactg	gattccaaag	ttctcaatgc	tgcttgctgt	tcttgaatgg	700
701	ggggtcgttg	ggcaataagg	gctgcacgcg	cacttttatc	cgcctctgct	750
751	gcgctccgcc	accgtacgta	aatttatggt	tggttatgaa	atgctggcag	800
801	agacccagcg	agacctgacc	gcagaacagg	cagcagagcg	tttgcgcgca	850
851	gtcagcgata	tccattttcg	cgaatccgga	gtgtaagaaa	tgagtctgaa	900
901	agaaaaaaca	caatctctgt	ttgccaacgc	atttggctac	cctgccactc	950
951	acaccattca	ggcgcctggc	cgcgtgaatt	tgattggtga	acacaccgac	1000
1001	tacaacgacg	gtttcgttct	gccctgcgcg	attgattatc	aaaccgtgat	1050
1051	cagttgtgca	ccacgcgatg	accgtaaagt	tcgcgtgatg	gcagccgatt	1100
1101	atgaaaatca	gctcgacgag	ttttccctcg	atgcgcccat	tgtcgcacat	1150
1151	gaaaactatc	aatgggctaa	ctacgttcgt	ggcgtggtga	aacatctgca	1200
1201	actgcgtaac	aacagcttcg	gcggcgtgga	catggtgatc	agcggcaatg	1250
1251	tgccgacggg	tgccgggtta	agttcttccg	cttcactgga	agtcgcggtc	1300
1301	ggaaccgtat	tgcagcagct	ttatcatctg	ccgctggacg	gcgcacaaat	1350
1351	cgcgcttaac	ggtcaggaag	cagaaaacca	gtttgtaggc	tgtaactgcg	1400
1401	ggatcatgga	tcagctaatt	tccgcgctcg	gcaagaaaga	tcatgccttg	1450
1451	ctgatcgatt	gccgctcact	ggggaccaaa	gcagtttcca	tgcccaaagg	1500
1501	tgtggctgtc	gtcatcatca	acagtaactt	caaacgtacc	ctggttggca	1550
1551	gcgaatacaa	cacccgtcgt	gaacagtgcg	aaaccggtgc	gcgtttcttc	1600
1601	cagcagccag	ccctgcgtga	tgtcaccatt	gaagagttca	acgctgttgc	1650
1651	gcatgaactg	gacccgatcg	tggcaaaacg	cgtgcgtcat	atactgactg	1700
1701	aaaacgcccg	caccgttgaa	gctgccagcg	cgctggagca	aggcgacctg	1750
1751	aaacgtatgg	gcgagttgat	ggcggagtct	catgcctcta	tgcgcgatga	1800
1801	tttcgaaatc	accgtgccgc	aaattgacac	tctggtagaa	atcgtcaaag	1850
1851	ctgtgattgg	cgacaaaggt	ggcgtacgca	tgaccggcgg	cggatttggc	1900
1901	ggctgtatcg	tcgcgctgat	cccggaagag	ctggtgcctg	ccgcacagca	1950
1951	agctgtcgct	gaacaatatg	aagcaaaaac	aggtattaaa	gagacttttt	2000
2001	acgtttgtaa	accatcacaa	ggagcaggac	agtgctgaac	gaaactcccg	2050
2051	cactggcacc	cgatggcagc	cgtaccgact	gttctgcctc	gcgcgtttcg	2100
2101	gtgatgacgg	tgaaaacctc	tgacacatgc	agctcccgga	gacggtcaca	2150
2151	gcttgtctgt	aagcggatgc	cgggagcaga	caagcccgtc	agggcgcgtc	2200
2201	agcgggtgtt	ggcgggtgtc	ggggcgcagc	catgacccag	tcacgtagcg	2250
2251	atagcggagt	gtatactggc	ttaactatgc	ggcatcagag	cagattgtac	2300
2301	tgagagtgca	ccatatgcgg	tgtgaaatac	cgcacagatg	cgtaaggaga	2350
2351	aaataccgca	tcaggcgctc	ttccgcttcc	tcgctcactg	actcgctgcg	2400
2401	ctcggtcgtt	cggctgcggc	gagcggtatc	agctcactca	aaggcggtaa	2450
2451	tacggttatc	cacagaatca	ggggataacg	caggaaagaa	catgtgagca	2500
2501	aaaggccagc	aaaaggccag	gaaccgtaaa	aaggccgcgt	tgctggcgtt	2550
2551	tttccatagg	ctccgccccc	ctgacgagca	tcacaaaaat	cgacgctcaa	2600
2601	gtcagaggtg	gcgaaacccg	acaggactat	aaagatacca	ggcgtttccc	2650
2651	cctggaagct	ccctcgtgcg	ctctcctgtt	ccgaccctgc	cgcttaccgg	2700
2701	atacctgtcc	gcctttctcc	cttcgggaag	cgtggcgctt	tctcatagct	2750
2751	cacgctgtag	gtatctcagt	tcggtgtagg	tcgttcgctc	caagctgggc	2800
2801	tgtgtgcacg	aaccccccgt	tcagcccgac	cgctgcgcct	tatccggtaa	2850
2851	ctatcgtctt	gagtccaacc	cggtaagaca	cgacttatcg	ccactggcag	2900
2901	cagccactgg	taacaggatt	agcagagcga	ggtatgtagg	cggtgctaca	2950
2951	gagttcttga	agtggtggcc	taactacggc	tacactagaa	ggacagtatt	3000
3001	tggtatctgc	gctctgctga	agccagttac	cttcggaaaa	agagttggta	3050
3051	gctcttgatc	cggcaaacaa	accaccgctg	gtagcggtgg	tttttttgtt	3100
3101	tgcaagcagc	agattacgcg	cagaaaaaaa	ggatctcaag	aagatccttt	3150
3151	gatcttttct	acggggtctg	acgctcagtg	gaacgaaaac	tcacgttaag	3200
3201	ggattttggt	catgagatta	tcaaaaagga	tcttcaccta	gatcctttta	3250
3251	aattaaaaat	gaagttttaa	atcaatctaa	agtatatatg	agtaaacttg	3300
3301	gtctgacagt	taccaatgct	taatcagtga	ggcacctatc	tcagcgatct	3350
3351	gtctatttcg	ttcatccata	gttgcctgac	tccccgtcgt	gtagataact	3400
3401	acgatacggg	agggcttacc	atctggcccc	agtgctgcaa	tgataccgcg	3450
3451	agacccacgc	tcaccggctc	cagatttatc	agcaataaac	cagccagccg	3500
3501	gaagggccga	gcgcagaagt	ggtcctgcaa	ctttatccgc	ctccatccag	3550
3551	tctattaatt	gttgccggga	agctagagta	agtagttcgc	cagttaatag	3600
3601	tttgcgcaac	gttgttgcca	ttgctgcagg	catcgtggtg	tcacgctcgt	3650
3651	cgtttggtat	ggcttcattc	agctccggtt	cccaacgatc	aaggcgagtt	3700
3701	acatgatccc	ccatgttgtg	caaaaaagcg	gttagctcct	tcggtcctcc	3750
3751	gatcgttgtc	agaagtaagt	tggccgcagt	gttatcactc	atggttatgg	3800
3801	cagcactgca	taattctctt	actgtcatgc	catccgtaag	atgcttttct	3850
3851	gtgactggtg	agtactcaac	caagtcattc	tgagaatagt	gtatgcggcg	3900
3901	accgagttgc	tcttgcccgg	cgtcaacacg	ggataatacc	gcgccacata	3950
3951	gcagaacttt	aaaagtgctc	atcattggaa	aacgttcttc	ggggcgaaaa	4000
4001	ctctcaagga	tcttaccgct	gttgagatcc	agttcgatgt	aacccactcg	4050
4051	tgcacccaac	tgatcttcag	catcttttac	tttcaccagc	gtttctgggt	4100
4101	gagcaaaaac	aggaaggcaa	aatgccgcaa	aaaagggaat	aagggcgaca	4150
4151	cggaaatgtt	gaatactcat	actcttcctt	tttcaatatt	attgaagcat	4200
4201	ttatcagggt	tattgtctca	tgagcggata	catatttgaa	tgtatttaga	4250
4251	aaaataaaca	aataggggtt	ccgcgcacat	ttccccgaaa	agtgccacct	4300
4301	gacgtctaag	aaaccattat	tatcatgaca	ttaacctata	aaaataggcg	4350
4351	tatcacgagg	ccctttcgtc	ttcaagaa	4378