pFastBacHT C（pFastBacHTC）

pFastBacHT C（pFastBacHTC）

质粒名称: pFastBacHT C
其他名称:
质粒大小（bp）: 4858
抗性: Ampicillin
质粒类型: Other
是否病毒: Baculoviral
启动子: polyhedrin (P-PH)
稳转或瞬转: Unspecified
组成型或诱导性: Constitutive
表达水平:
蛋白标签: His (Nterm)
测序引物:
GenBank号:
其他属性: 5′ Sequencing 1 Primer:Polyhedrin
5′ Sequencing 1 Primer Sequence:5’d[AAATGATAACCATCTCGC]3′
Selectable Marker:Gentamicin
Notes:

Purification of his-tagged proteins from insect cells

质粒图谱:

质粒序列:

1	GACGCGCCCT	GTAGCGGCGC	ATTAAGCGCG	GCGGGTGTGG	TGGTTACGCG	50
51	CAGCGTGACC	GCTACACTTG	CCAGCGCCCT	AGCGCCCGCT	CCTTTCGCTT	100
101	TCTTCCCTTC	CTTTCTCGCC	ACGTTCGCCG	GCTTTCCCCG	TCAAGCTCTA	150
151	AATCGGGGGC	TCCCTTTAGG	GTTCCGATTT	AGTGCTTTAC	GGCACCTCGA	200
201	CCCCAAAAAA	CTTGATTAGG	GTGATGGTTC	ACGTAGTGGG	CCATCGCCCT	250
251	GATAGACGGT	TTTTCGCCCT	TTGACGTTGG	AGTCCACGTT	CTTTAATAGT	300
301	GGACTCTTGT	TCCAAACTGG	AACAACACTC	AACCCTATCT	CGGTCTATTC	350
351	TTTTGATTTA	TAAGGGATTT	TGCCGATTTC	GGCCTATTGG	TTAAAAAATG	400
401	AGCTGATTTA	ACAAAAATTT	AACGCGAATT	TTAACAAAAT	ATTAACGTTT	450
451	ACAATTTCAG	GTGGCACTTT	TCGGGGAAAT	GTGCGCGGAA	CCCCTATTTG	500
501	TTTATTTTTC	TAAATACATT	CAAATATGTA	TCCGCTCATG	AGACAATAAC	550
551	CCTGATAAAT	GCTTCAATAA	TATTGAAAAA	GGAAGAGTAT	GAGTATTCAA	600
601	CATTTCCGTG	TCGCCCTTAT	TCCCTTTTTT	GCGGCATTTT	GCCTTCCTGT	650
651	TTTTGCTCAC	CCAGAAACGC	TGGTGAAAGT	AAAAGATGCT	GAAGATCAGT	700
701	TGGGTGCACG	AGTGGGTTAC	ATCGAACTGG	ATCTCAACAG	CGGTAAGATC	750
751	CTTGAGAGTT	TTCGCCCCGA	AGAACGTTTT	CCAATGATGA	GCACTTTTAA	800
801	AGTTCTGCTA	TGTGGCGCGG	TATTATCCCG	TATTGACGCC	GGGCAAGAGC	850
851	AACTCGGTCG	CCGCATACAC	TATTCTCAGA	ATGACTTGGT	TGAGTACTCA	900
901	CCAGTCACAG	AAAAGCATCT	TACGGATGGC	ATGACAGTAA	GAGAATTATG	950
951	CAGTGCTGCC	ATAACCATGA	GTGATAACAC	TGCGGCCAAC	TTACTTCTGA	1000
1001	CAACGATCGG	AGGACCGAAG	GAGCTAACCG	CTTTTTTGCA	CAACATGGGG	1050
1051	GATCATGTAA	CTCGCCTTGA	TCGTTGGGAA	CCGGAGCTGA	ATGAAGCCAT	1100
1101	ACCAAACGAC	GAGCGTGACA	CCACGATGCC	TGTAGCAATG	GCAACAACGT	1150
1151	TGCGCAAACT	ATTAACTGGC	GAACTACTTA	CTCTAGCTTC	CCGGCAACAA	1200
1201	TTAATAGACT	GGATGGAGGC	GGATAAAGTT	GCAGGACCAC	TTCTGCGCTC	1250
1251	GGCCCTTCCG	GCTGGCTGGT	TTATTGCTGA	TAAATCTGGA	GCCGGTGAGC	1300
1301	GTGGGTCTCG	CGGTATCATT	GCAGCACTGG	GGCCAGATGG	TAAGCCCTCC	1350
1351	CGTATCGTAG	TTATCTACAC	GACGGGGAGT	CAGGCAACTA	TGGATGAACG	1400
1401	AAATAGACAG	ATCGCTGAGA	TAGGTGCCTC	ACTGATTAAG	CATTGGTAAC	1450
1451	TGTCAGACCA	AGTTTACTCA	TATATACTTT	AGATTGATTT	AAAACTTCAT	1500
1501	TTTTAATTTA	AAAGGATCTA	GGTGAAGATC	CTTTTTGATA	ATCTCATGAC	1550
1551	CAAAATCCCT	TAACGTGAGT	TTTCGTTCCA	CTGAGCGTCA	GACCCCGTAG	1600
1601	AAAAGATCAA	AGGATCTTCT	TGAGATCCTT	TTTTTCTGCG	CGTAATCTGC	1650
1651	TGCTTGCAAA	CAAAAAAACC	ACCGCTACCA	GCGGTGGTTT	GTTTGCCGGA	1700
1701	TCAAGAGCTA	CCAACTCTTT	TTCCGAAGGT	AACTGGCTTC	AGCAGAGCGC	1750
1751	AGATACCAAA	TACTGTCCTT	CTAGTGTAGC	CGTAGTTAGG	CCACCACTTC	1800
1801	AAGAACTCTG	TAGCACCGCC	TACATACCTC	GCTCTGCTAA	TCCTGTTACC	1850
1851	AGTGGCTGCT	GCCAGTGGCG	ATAAGTCGTG	TCTTACCGGG	TTGGACTCAA	1900
1901	GACGATAGTT	ACCGGATAAG	GCGCAGCGGT	CGGGCTGAAC	GGGGGGTTCG	1950
1951	TGCACACAGC	CCAGCTTGGA	GCGAACGACC	TACACCGAAC	TGAGATACCT	2000
2001	ACAGCGTGAG	CATTGAGAAA	GCGCCACGCT	TCCCGAAGGG	AGAAAGGCGG	2050
2051	ACAGGTATCC	GGTAAGCGGC	AGGGTCGGAA	CAGGAGAGCG	CACGAGGGAG	2100
2101	CTTCCAGGGG	GAAACGCCTG	GTATCTTTAT	AGTCCTGTCG	GGTTTCGCCA	2150
2151	CCTCTGACTT	GAGCGTCGAT	TTTTGTGATG	CTCGTCAGGG	GGGCGGAGCC	2200
2201	TATGGAAAAA	CGCCAGCAAC	GCGGCCTTTT	TACGGTTCCT	GGCCTTTTGC	2250
2251	TGGCCTTTTG	CTCACATGTT	CTTTCCTGCG	TTATCCCCTG	ATTCTGTGGA	2300
2301	TAACCGTATT	ACCGCCTTTG	AGTGAGCTGA	TACCGCTCGC	CGCAGCCGAA	2350
2351	CGACCGAGCG	CAGCGAGTCA	GTGAGCGAGG	AAGCGGAAGA	GCGCCTGATG	2400
2401	CGGTATTTTC	TCCTTACGCA	TCTGTGCGGT	ATTTCACACC	GCAGACCAGC	2450
2451	CGCGTAACCT	GGCAAAATCG	GTTACGGTTG	AGTAATAAAT	GGATGCCCTG	2500
2501	CGTAAGCGGG	TGTGGGCGGA	CAATAAAGTC	TTAAACTGAA	CAAAATAGAT	2550
2551	CTAAACTATG	ACAATAAAGT	CTTAAACTAG	ACAGAATAGT	TGTAAACTGA	2600
2601	AATCAGTCCA	GTTATGCTGT	GAAAAAGCAT	ACTGGACTTT	TGTTATGGCT	2650
2651	AAAGCAAACT	CTTCATTTTC	TGAAGTGCAA	ATTGCCCGTC	GTATTAAAGA	2700
2701	GGGGCGTGGC	CAAGGGCATG	GTAAAGACTA	TATTCGCGGC	GTTGTGACAA	2750
2751	TTTACCGAAC	AACTCCGCGG	CCGGGAAGCC	GATCTCGGCT	TGAACGAATT	2800
2801	GTTAGGTGGC	GGTACTTGGG	TCGATATCAA	AGTGCATCAC	TTCTTCCCGT	2850
2851	ATGCCCAACT	TTGTATAGAG	AGCCACTGCG	GGATCGTCAC	CGTAATCTGC	2900
2901	TTGCACGTAG	ATCACATAAG	CACCAAGCGC	GTTGGCCTCA	TGCTTGAGGA	2950
2951	GATTGATGAG	CGCGGTGGCA	ATGCCCTGCC	TCCGGTGCTC	GCCGGAGACT	3000
3001	GCGAGATCAT	AGATATAGAT	CTCACTACGC	GGCTGCTCAA	ACCTGGGCAG	3050
3051	AACGTAAGCC	GCGAGAGCGC	CAACAACCGC	TTCTTGGTCG	AAGGCAGCAA	3100
3101	GCGCGATGAA	TGTCTTACTA	CGGAGCAAGT	TCCCGAGGTA	ATCGGAGTCC	3150
3151	GGCTGATGTT	GGGAGTAGGT	GGCTACGTCT	CCGAACTCAC	GACCGAAAAG	3200
3201	ATCAAGAGCA	GCCCGCATGG	ATTTGACTTG	GTCAGGGCCG	AGCCTACATG	3250
3251	TGCGAATGAT	GCCCATACTT	GAGCCACCTA	ACTTTGTTTT	AGGGCGACTG	3300
3301	CCCTGCTGCG	TAACATCGTT	GCTGCTGCGT	AACATCGTTG	CTGCTCCATA	3350
3351	ACATCAAACA	TCGACCCACG	GCGTAACGCG	CTTGCTGCTT	GGATGCCCGA	3400
3401	GGCATAGACT	GTACAAAAAA	ACAGTCATAA	CAAGCCATGA	AAACCGCCAC	3450
3451	TGCGCCGTTA	CCACCGCTGC	GTTCGGTCAA	GGTTCTGGAC	CAGTTGCGTG	3500
3501	AGCGCATACG	CTACTTGCAT	TACAGTTTAC	GAACCGAACA	GGCTTATGTC	3550
3551	AACTGGGTTC	GTGCCTTCAT	CCGTTTCCAC	GGTGTGCGTC	ACCCGGCAAC	3600
3601	CTTGGGCAGC	AGCGAAGTCG	AGGCATTTCT	GTCCTGGCTG	GCGAACGAGC	3650
3651	GCAAGGTTTC	GGTCTCCACG	CATCGTCAGG	CATTGGCGGC	CTTGCTGTTC	3700
3701	TTCTACGGCA	AGGTGCTGTG	CACGGATCTG	CCCTGGCTTC	AGGAGATCGG	3750
3751	AAGACCTCGG	CCGTCGCGGC	GCTTGCCGGT	GGTGCTGACC	CCGGATGAAG	3800
3801	TGGTTCGCAT	CCTCGGTTTT	CTGGAAGGCG	AGCATCGTTT	GTTCGCCCAG	3850
3851	GACTCTAGCT	ATAGTTCTAG	TGGTTGGCTA	CGTATACTCC	GGAATATTAA	3900
3901	TAGATCATGG	AGATAATTAA	AATGATAACC	ATCTCGCAAA	TAAATAAGTA	3950
3951	TTTTACTGTT	TTCGTAACAG	TTTTGTAATA	AAAAAACCTA	TAAATATTCC	4000
4001	GGATTATTCA	TACCGTCCCA	CCATCGGGCG	CGGATCTCGG	TCCGAAACCA	4050
4051	TGTCGTACTA	CCATCACCAT	CACCATCACG	ATTACGATAT	CCCAACGACC	4100
4101	GAAAACCTGT	ATTTTCAGGG	CGCCATGGGG	ATCCGGAATT	CAAAGGCCTA	4150
4151	CGTCGACGAG	CTCACTAGTC	GCGGCCGCTT	TCGAATCTAG	AGCCTGCAGT	4200
4201	CTCGAGGCAT	GCGGTACCAA	GCTTGTCGAG	AAGTACTAGA	GGATCATAAT	4250
4251	CAGCCATACC	ACATTTGTAG	AGGTTTTACT	TGCTTTAAAA	AACCTCCCAC	4300
4301	ACCTCCCCCT	GAACCTGAAA	CATAAAATGA	ATGCAATTGT	TGTTGTTAAC	4350
4351	TTGTTTATTG	CAGCTTATAA	TGGTTACAAA	TAAAGCAATA	GCATCACAAA	4400
4401	TTTCACAAAT	AAAGCATTTT	TTTCACTGCA	TTCTAGTTGT	GGTTTGTCCA	4450
4451	AACTCATCAA	TGTATCTTAT	CATGTCTGGA	TCTGATCACT	AGATCTGCCT	4500
4501	AGGAGATCCG	AACCAGATAA	GTGAAATCTA	GTTCCAAACT	ATTTTGTCAT	4550
4551	TTTTAATTTT	CGTATTAGCT	TACGACGCTA	CACCCAGTTC	CCATCTATTT	4600
4601	TGTCACTCTT	CCCTAAATAA	TCCTTAAAAA	CTCCATTTCC	ACCCCTCCCA	4650
4651	GTTCCCAACT	ATTTTGTCCG	CCCACAGCGG	GGCATTTTTC	TTCCTGTTAT	4700
4701	GTTTTTAATC	AAACATCCTG	CCAACTCCAT	GTGACAAACC	GTCATCTTCG	4750
4751	GCTACTTTTT	CTCTGTCACA	GAATGAAAAT	TTTTCTGTCA	TCTCTTCGTT	4800
4801	ATTAATGTTT	GTAATTGACT	GAATATCAAC	GCTTATTTGC	AGCCTGAATG	4850
4851	GCGAATGG	4858