手机验证
-20℃
6个月
pFastBacHT C
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pFastBacHTC, pFastBac HTC, pFastBacHT C, pFastBac HT C |
质粒类型: | 昆虫细胞表达载体 |
启动子: | Polyhedrin |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4858 bp |
5' 测序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
3' 测序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
载体标签: | N-His, N-TEV |
载体抗性: | Ampicillin 氨苄 |
筛选标记: | Gentamicin 庆大霉素 |
备注: | |
稳定性: | 稳表达 Stable |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 杆状病毒 |
The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag.
• Strong polyhedrin (PH) promoter for high-level protein expression.
• N-terminal 6xHis tag for purification of recombinant fusion proteins using metal-chelating resin and a TEV protease cleavage site for removal of the 6xHis tagfollowing protein purification.
• Vector supplied in 3 reading frames for simplified cloning.
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