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- 详细信息
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pFastBacHT C
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pFastBacHT C
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4858
/invitrogen="608"
/vntifkey="98"
CDS 589..1449
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
promoter 490..588
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
rep_origin 2..457
/invitrogen="2520000"
/vntifkey="33"
/label=f1\origin
CDS complement(2802..3335)
/invitrogen="1150000"
/vntifkey="4"
/label=Gm(R)
misc_feature 4119..4224
/invitrogen="1550000"
/vntifkey="21"
/label=MCS
promoter complement(3524..3551)
/invitrogen="2250000"
/vntifkey="29"
/label=Pc\promoter
promoter 3904..4032
/invitrogen="2260000"
/vntifkey="29"
/label=PH\promoter
primer 3920..3937
/invitrogen="2060000"
/vntifkey="27"
/label=Polyhedrin\forward\primer
rep_origin 1594..2267
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
polyA_signal 4242..4482
/invitrogen="1850000"
/vntifkey="25"
/label=SV40\pA
CDS 4101..4121
/invitrogen="1300000"
/vntifkey="4"
/label=TEV\recognition\site
misc_recomb 4501..4695
/invitrogen="2750000"
/vntifkey="86"
/label=Tn7L
misc_recomb 2512..2735
/invitrogen="2760000"
/vntifkey="86"
/label=Tn7R
CDS 4062..4079
/invitrogen="1015000"
/vntifkey="4"
/label=6xHis
CDS 4050..4052
/invitrogen="123000000"
/vntifkey="4"
/label=initiation\ATG
BASE COUNT 1219 a 1198 c 1153 g 1288 t
ORIGIN
1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatgggg atccggaatt
4141 caaaggccta cgtcgacgag ctcactagtc gcggccgctt tcgaatctag agcctgcagt
4201 ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga ggatcataat cagccatacc
4261 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
4321 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
4381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
4441 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact agatctgcct
4501 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
4561 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
4621 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
4681 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
4741 gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt
4801 attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatgg
pFastBacHTC载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pFastBacHTC, pFastBac HTC, pFastBacHT C, pFastBac HT C |
| 质粒类型: | 昆虫细胞表达载体 |
| 启动子: | Polyhedrin |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4858 bp |
| 5' 测序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
| 3' 测序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
| 载体标签: | N-His, N-TEV |
| 载体抗性: | Ampicillin 氨苄 |
| 筛选标记: | Gentamicin 庆大霉素 |
| 备注: | |
| 稳定性: | 稳表达 Stable |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 杆状病毒 |
pFastBacHTC载体质粒图谱和多克隆位点信息
pFastBacHTC载体简介
The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag.
• Strong polyhedrin (PH) promoter for high-level protein expression.
• N-terminal 6xHis tag for purification of recombinant fusion proteins using metal-chelating resin and a TEV protease cleavage site for removal of the 6xHis tagfollowing protein purification.
• Vector supplied in 3 reading frames for simplified cloning.
pFastBacHTC载体序列
LOCUS pFastBacTHT\C 4858 bp DNA circular SYN 2-SEP-2003SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4858
/invitrogen="608"
/vntifkey="98"
CDS 589..1449
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
promoter 490..588
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
rep_origin 2..457
/invitrogen="2520000"
/vntifkey="33"
/label=f1\origin
CDS complement(2802..3335)
/invitrogen="1150000"
/vntifkey="4"
/label=Gm(R)
misc_feature 4119..4224
/invitrogen="1550000"
/vntifkey="21"
/label=MCS
promoter complement(3524..3551)
/invitrogen="2250000"
/vntifkey="29"
/label=Pc\promoter
promoter 3904..4032
/invitrogen="2260000"
/vntifkey="29"
/label=PH\promoter
primer 3920..3937
/invitrogen="2060000"
/vntifkey="27"
/label=Polyhedrin\forward\primer
rep_origin 1594..2267
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
polyA_signal 4242..4482
/invitrogen="1850000"
/vntifkey="25"
/label=SV40\pA
CDS 4101..4121
/invitrogen="1300000"
/vntifkey="4"
/label=TEV\recognition\site
misc_recomb 4501..4695
/invitrogen="2750000"
/vntifkey="86"
/label=Tn7L
misc_recomb 2512..2735
/invitrogen="2760000"
/vntifkey="86"
/label=Tn7R
CDS 4062..4079
/invitrogen="1015000"
/vntifkey="4"
/label=6xHis
CDS 4050..4052
/invitrogen="123000000"
/vntifkey="4"
/label=initiation\ATG
BASE COUNT 1219 a 1198 c 1153 g 1288 t
ORIGIN
1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatgggg atccggaatt
4141 caaaggccta cgtcgacgag ctcactagtc gcggccgctt tcgaatctag agcctgcagt
4201 ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga ggatcataat cagccatacc
4261 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
4321 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
4381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
4441 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact agatctgcct
4501 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
4561 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
4621 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
4681 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
4741 gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt
4801 attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatgg
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pFastBacHT C
¥800 - 1200
